Isolation and Characterization of Live Yeast Cells from Ancient Clay Vessels
Authors: Tzemach Aouizerat,
Aren M. Maeir,
Yitzhak Paz,
Yuval Gadot,
Amir Szitenberg,
Sivan Alkalay-Oren,
Shunit Coppenhagen-Glazer,
Michael Klutstein and
Ronen Hazan,
date: 01/05/2020,
view: 456,
Q&A: 0
[Abstract] Ancient fermented food has been studied mainly based on residue analysis and recipes and reconstruction attempts were performed using modern domesticated yeast. Furthermore, microorganisms which participated in fermentation were studied using ancient-DNA techniques. In a recent paper, we presented a novel approach based on the hypothesis that ...
Acute Isolation of Cells from Murine Sino-atrial Node
Authors: Qadeer Aziz,
Muriel Nobles and
Andrew Tinker,
date: 01/05/2020,
view: 340,
Q&A: 0
[Abstract] The cardiac conduction system allows the synchronized propagation of electrical activity through heart muscle. This is initiated by the spontaneous activity of the specialized pacemaker cells of the sino-atrial node (SAN). The SAN region underlies automaticity in mammals and therefore has a crucial role in the pathogenesis of cardiac disorders ...
In vitro Differentiation of Human iPSC-derived Retinal Pigment Epithelium Cells (iPSC-RPE)
[Abstract] Induced Pluripotent Stem Cells (iPSCs) serve as an excellent model system for studying the molecular underpinnings of tissue development. Human iPSC-derived retinal pigment epithelium (iPSC-RPE) cells have fetal-like molecular profiles. Hence, biobanks like iPSCORE, which contain iPSCs generated from hundreds of individuals, are an invaluable ...
An Optimized Method to Isolate Human Fibroblasts from Tissue for Ex Vivo Analysis
Authors: Sara Waise,
Rachel Parker,
Matthew J. J. Rose-Zerilli,
David M. Layfield,
Oliver Wood,
Jonathan West,
Christian H. Ottensmeier,
Gareth J. Thomas and
Christopher J. Hanley,
date: 12/05/2019,
view: 615,
Q&A: 0
[Abstract] Despite their involvement in many physiological and pathological processes, fibroblasts remain a poorly-characterized cell type. Analysis of primary fibroblasts while maintaining their in vivo phenotype is challenging: standard methods for fibroblast isolation require cell culture in vitro, which is known to alter phenotypes. ...
Opto-magnetic Selection and Isolation of Single Cells
Authors: Loïc Binan,
Joannie Roy and
Santiago Costantino,
date: 11/20/2019,
view: 491,
Q&A: 0
[Abstract] Capturing single cells from large heterogenous populations based solely on observable traits is necessary for many cell biology applications and remains a major technical challenge. The protocol we present allows the isolation of viable and metabolically active cells selected for their shape, migration speed, contact to other cells, or ...
Isolation and Culture of Murine Hepatic Stellate Cells
Authors: Rucha V. Modak and
Dietmar M. Zaiss,
date: 11/05/2019,
view: 780,
Q&A: 0
[Abstract] Hepatic stellate cells (HSCs), alternatively known as liver pericytes, can differentiate into myofibroblasts and secrete extra-cellular matrix components, thereby promoting wound healing and fibrosis. Studying HSCs can provide insights into the pathological mechanisms governing these processes. HSC isolation methods typically comprise of enzymatic ...