Cell isolation and culture —Cell Biology

Hair Follicle Stem Cell Isolation and Expansion

Authors: Mindy Call, Ewa Anna Meyer, Winston W. Kao, Friedrich E. Kruse and Ursula Schlӧtzer-Schrehardt, date: 05/20/2018, view: 50, Q&A: 0

[Abstract] Stem cells are widely used for numerous clinical applications including limbal stem cell deficiency. Stem cell derived from the bulge region of the hair follicle have the ability to differentiate into a variety of cell types including interfollicular epidermis, hair follicle structures, sebaceous glands and corneal epithelial cells when provided ...

Cobblestone Area-forming Cell Assay of Mouse Bone Marrow Hematopoietic Stem Cells

Authors: Surya Amarachintha and Qishen Pang, date: 05/05/2018, view: 119, Q&A: 0

[Abstract] Bone Marrow Hematopoietic Stem Cells (HSCs) require bone marrow microenvironment for their maintenance and proliferation. Culture of Bone Marrow Mesenchymal Stromal Cells (MSCs) provides appropriate environmental signals for HSCs survival in vitro. Here, we provide a detailed protocol that describes culture conditions for MSCs, flow ...

3D Co-culture System of Tumor-associated Macrophages and Ovarian Cancer Cells

Authors: Lingli Long, Mingzhu Yin and Wang Min, date: 04/20/2018, view: 453, Q&A: 0

[Abstract] Ovarian cancer is fairly unique in that ovarian carcinoma cells can detach and spread directly through peritoneal cavity. It has been unclear, however, how detached cancer cells survive in the peritoneum and form spheroid structure. We have recently reported that there is a strong correlation between Tumor-associated macrophages (TAMs)-associated ...

Alphavirus Purification Using Low-speed Spin Centrifugation

Authors: Vamseedhar Rayaprolu, Jolene Ramsey, Joseph Che-Yen Wang and Suchetana Mukhopadhyay, date: 03/20/2018, view: 419, Q&A: 0

[Abstract] Chemical and sedimentation procedures are used to purify virus particles. While these approaches are successful for wild-type viruses, they are often not feasible for purifying mutant viruses with assembly defects. We combined two published methods (Atasheva et al., 2013; Moller-Tank et al., 2013), to generate a protocol that ...

Microbial Mutagenicity Assay: Ames Test

Authors: Urvashi Vijay, Sonal Gupta, Priyanka Mathur, Prashanth Suravajhala and Pradeep Bhatnagar, date: 03/20/2018, view: 779, Q&A: 0

[Abstract] The Microbial mutagenicity Ames test is a bacterial bioassay accomplished in vitro to evaluate the mutagenicity of various environmental carcinogens and toxins. While Ames test is used to identify the revert mutations which are present in strains, it can also be used to detect the mutagenicity of environmental samples such as drugs, dyes, ...

Isolation and Establishment of Mesenchymal Stem Cells from Wharton’s Jelly of Human Umbilical Cord

Authors: Umesh Goyal, Chitra Jaiswal and Malancha Ta, date: 02/20/2018, view: 763, Q&A: 3

[Abstract] Mesenchymal stem cells (MSCs) are currently considered as ‘medicinal signaling cells’ and a promising resource in regard to cell-based regenerative therapy. Umbilical cord is a human term perinatal tissue which is easily attainable, and a promising source of stem cells with no associated ethical concerns. MSCs have been isolated from different ...

Sebinger Culture: A System Optimized for Morphological Maturation and Imaging of Cultured Mouse Metanephric Primordia

Authors: Mona Elhendawi and Jamie A. Davies, date: 02/20/2018, view: 509, Q&A: 0

[Abstract] Here, we present a detailed protocol on setting up embryonic renal organ cultures using a culture method that we have optimised for anatomical maturation and imaging. Our culture method places kidney rudiments on glass in a thin film of medium, which results in very flat cultures with all tubules in the same image plane. For reasons not yet ...

Preparation of Amyloid Fibril Networks

Authors: Mirren Charnley , Jay Gilbert, Owen G. Jones and Nicholas P. Reynolds, date: 02/20/2018, view: 481, Q&A: 0

[Abstract] Networks of amyloid nanofibrils fabricated from common globular proteins such as lysozyme and β-lactoglobulin have material properties that mimic the extracellular microenvironment of many cell types. Cells cultured on such amyloid fibril networks show improved attachment, spreading and in the case of mesenchymal stem cells improved ...

Culture and Nucleofection of Postnatal Day 7 Cortical and Cerebellar Mouse Astroglial Cells

Authors: Malek Chouchane and Marcos Romualdo Costa, date: 02/05/2018, view: 515, Q&A: 0

[Abstract] Lineage reprogramming of astroglial cells isolated from different brain regions leads to the generation of different neuronal subtypes. This protocol describes the isolation and culture of neocortical and cerebellar astrocytes from postnatal mice. We also present a comprehensive description of the main steps towards successful gene delivery in ...

Generation of Chemically Induced Liver Progenitors (CLiPs) from Rat Adult Hepatocytes

Authors: Takeshi Katsuda, Kazunori Hosaka and Takahiro Ochiya, date: 01/20/2018, view: 835, Q&A: 0

[Abstract] Primary mature hepatocytes (MHs) or their progenitor cells are candidate cell sources for cell transplantation therapy in severe liver diseases. However, stable culture of these cells or generation of equivalent cells from pluripotent stem cells has been limited. Using a cocktail of small molecules that we previously found useful in stable culture ...

Cell Biology

Ex vivo Follicle Rupture and in situ Zymography in Drosophila

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