Generation of Human Mesenchymal Stem Cell 3D Spheroids Using Low-binding Plates
[Abstract] The 3D culture of human mesenchymal stem cells (hMSCs) represents a more physiological environment than classical 2D culture and has been used to enhance the MSC secretome or extend cell survival after transplantation. Here we describe a simple and affordable method to generate 3D spheroids of hMSCs by seeding them at high density in a low-binding ...
Isolation of LYVE-1+ Endothelial Cells from Mouse Embryos
Author: Patrick Crosswhite,
date: 08/05/2018,
view: 117,
Q&A: 0
[Abstract] Lymphatic vessel endothelial hyaluronan receptor 1, or LYVE-1, is a type 1 integral membrane glycoprotein expressed by lymphatic endothelial cells (LECs). LYVE-1 is commonly used as a biological marker to visually distinguish developing lymphatic vessels from blood endothelial cells (arteries or veins). As our understanding of lymphatic biology is ...
Measuring Secretion of Capsidiol in Leaf Tissues of Nicotiana benthamiana
[Abstract] Plant species produce a wide variety of antimicrobial metabolites to protect themselves against potential pathogens in natural environments. Phytoalexins are low molecular weight compounds produced by plants in response to attempted attacks of pathogens. Accumulation of phytoalexins in attacked plant tissues can inhibit the growth of penetrating ...
Long-term in vitro Culture of Cryptosporidium parvum
Authors: Nigel Yarlett and
Mary Morada,
date: 08/05/2018,
view: 128,
Q&A: 0
[Abstract] Continuous in vitro growth of Cryptosporidium parvum has proved difficult and conventional in vitro culture techniques result in short-term (2-5 days) growth of the parasite resulting in thin-walled oocysts that fail to propagate using in vitro cultures, and do not produce an active infection using ...
Isolation of Microvascular Endothelial Cells
[Abstract] The vascular endothelium is essential to normal vascular homeostasis. Its dysfunction participates in various cardiovascular disorders. Murine endothelial cell culture is an important tool for cardiovascular disease research. This protocol demonstrates a quick, efficient method for the isolation of microvascular endothelial cells from murine ...
In vitro Osteoclastogenesis Assays Using Primary Mouse Bone Marrow Cells
Authors: Xueqian Zhuang and
Guohong Hu,
date: 06/05/2018,
view: 569,
Q&A: 1
[Abstract] Osteoclasts are a group of bone-absorbing cells to degenerate bone matrix and play pivotal roles in bone growth and homeostasis. The unbalanced induction of osteoclast differentiation (osteoclastogenesis) in pathological conditions, such as osteoporosis, arthritis and skeleton metastasis of cancer, causes great pain, bone fracture, hypercalcemia ...
Expansion of Airway Basal Cells and Generation of Polarized Epithelium
[Abstract] Airway basal stem cells are the progenitor cells within the airway that exhibit the capacity to self-renew and give rise to multiple types of differentiated airway epithelial cells. This stem cell-derived epithelium displays organized architecture with functional attributes of the airway mucosa. A protocol has been developed to culture and expand ...