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A Robust Mammary Organoid System to Model Lactation and Involution-like Processes

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A Pulse–chase EdU Method for Detection of Cell Division Orientation in Arabidopsis and Juncus prismatocarpus Leaf Primordia

Authors: Xiaofeng Yin and Hirokazu Tsukaya, date: 01/05/2021, view: 693, Q&A: 0

[Abstract] In plants, the morphological diversity of leaves is largely determined by cell division, especially cell division orientation. Whereas cell division itself is easily monitored, the detection and quantification of cell division orientation are difficult. The few existing methods for detection and quantification of cell division orientation are ...

Lipid Droplet Isolation from Arabidopsis thaliana Leaves

Authors: Yovanny Izquierdo, Rubén Fernández-Santos, Tomás Cascón and Carmen Castresana, date: 12/20/2020, view: 946, Q&A: 0

[Abstract]

Lipid droplets (LDs) are neutral lipid aggregates surrounded by a phospholipid monolayer and specific proteins. In plants, they play a key role as energy source after seed germination, but are also formed in vegetative tissues in response to developmental or environmental conditions, where their functions are poorly understood. To elucidate these,

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Acidified Blue Ink-staining Procedure for the Observation of Fungal Structures Inside Roots of Two Disparate Plant Lineages

Authors: Jill Kowal, Elena Arrigoni and Sophie Lane, date: 10/20/2020, view: 818, Q&A: 0

[Abstract] Identifying microscopic mycorrhizal fungal structures in roots, i.e., hyphae, vesicles and arbuscules, requires root staining procedures that are often time consuming and involves chemicals known to present health risks from exposure. By modifying established protocols, our root staining method stains roots using a safe ink- and ...

Multitarget Immunohistochemistry for Confocal and Super-resolution Imaging of Plant Cell Wall Polysaccharides

Authors: Kalina T. Haas, Methieu Rivière, Raymond Wightman and Alexis Peaucelle, date: 10/05/2020, view: 1727, Q&A: 0

[Abstract] The plant cell wall (PCW) is a pecto-cellulosic extracellular matrix that envelopes the plant cell. By integrating extra-and intra-cellular cues, PCW mediates a plethora of essential physiological functions. Notably, it permits controlled and oriented tissue growth by tuning its local mechano-chemical properties. To refine our knowledge of these ...

Live Cell Imaging of Male Meiosis in Arabidopsis by a Landmark-based System

Authors: Maria Ada Prusicki, Emma Mathilde Keizer, Rik Peter van Rosmalen, Christian Fleck and Arp Schnittger, date: 05/05/2020, view: 2463, Q&A: 0

[Abstract] Live cell imaging has tremendously promoted our understanding of cellular and subcellular processes such as cell division. Here, we present a step-by-step protocol for a robust and easy-to-use live cell imaging approach to study male meiosis in the plant Arabidopsis thaliana as recently established. Our method relies on the concomitant ...

Quantification of Protein Enrichment at Plasmodesmata

Authors: Magali S. Grison, Jules D. Petit, Marie Glavier and Emmanuelle M. Bayer, date: 03/05/2020, view: 1876, Q&A: 0

[Abstract] Intercellular communication plays a crucial role in the establishment of multicellular organisms by organizing and coordinating growth, development and defence responses. In plants, cell-to-cell communication takes place through nanometric membrane channels called plasmodesmata (PD). Understanding how PD dictate cellular connectivity greatly ...

Tandem Tag Assay Optimized for Semi-automated in vivo Autophagic Activity Measurement in Arabidopsis thaliana roots

Authors: Adrian N. Dauphinee, Jonas A. Ohlsson and Elena A. Minina, date: 03/05/2020, view: 2084, Q&A: 0

[Abstract] Autophagy is the main catabolic process in eukaryotes and plays a key role in cell homeostasis. In vivo measurement of autophagic activity (flux) is a powerful tool for investigating the role of the pathway in organism development and stress responses. Here we describe a significant optimization of the tandem tag assay for detection of ...

Visualization of Actin Organization and Quantification in Fixed Arabidopsis Pollen Grains and Tubes

Authors: Xiaolu Qu, Qiannan Wang, Haiyan Wang and Shanjin Huang, date: 01/05/2020, view: 2029, Q&A: 0

[Abstract] Although it is widely accepted that actin plays an important role in regulating pollen germination and pollen tube growth, how actin exactly performs functions remains incompletely understood. As the function of actin is dictated by its spatial organization, it is the key to reveal how exactly actin distributes in space in pollen cells. Here we ...

Analysis of Monosaccharides from Arabidopsis Seed Mucilage and Whole Seeds Using HPAEC-PAD

Authors: Gillian H. Dean, Kresimir Sola, Faride Unda, Shawn D. Mansfield and George W. Haughn, date: 12/20/2019, view: 2454, Q&A: 0

[Abstract] Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development. When mature seeds are hydrated, mucilage extrudes to form a gelatinous capsule around the seed. Determining the monosaccharide composition of ...

Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy

Authors: Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche, date: 08/20/2019, view: 2586, Q&A: 0

[Abstract] All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the cell and its surroundings. Despite its name, the cell wall is a flexible, dynamic structure. ...

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