• Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Past Issues in 2019

Volume: 9 Issue: 14

Biochemistry

Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina Authors:  Sowmya Bekshe Lokappa, M. Carter Cornwall and Jeannie Chen, date: 07/20/2019, view: 1167, Q&A: 0
Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, reduces its ability to activate the visual G-protein transducin. Binding of light-activated, ...
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In vitro RNA Cleavage Assays to Characterize IRE1-dependent RNA Decay Authors:  G. Elif Karagöz, Jirka Peschek, Peter Walter and Diego Acosta-Alvear, date: 07/20/2019, view: 872, Q&A: 0
The kinase/RNase IRE1 is a key effector of the cellular response to endoplasmic reticulum stress. The RNase activity of IRE1 can be measured in cells or in the test tube. Here we describe a protocol for the in vitro cleavage and analysis of RNA substrates of IRE1. The method consists of the in vitro transcription, purification ...
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Cancer Biology

Total RNA Isolation from Separately Established Monolayer and Hydrogel Cultures of Human Glioblastoma Cell Line Authors:  Manasi P Jogalekar and Elba E Serrano, date: 07/20/2019, view: 673, Q&A: 0
Astrocytoma is an invasive carcinoma occurring in the nervous system and currently lacks effective treatment options. A deeper understanding of the mechanisms of tumorigenesis and tumor progression is needed in order to develop novel therapeutic strategies. Recent advances in in vitro culture systems have demonstrated that the use of ...
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Evaluation of Genotoxicity by Micronucleus Assay in vitro and by Allium cepa Test in vivo Authors:  Christina N. Banti and Sotiris K. Hadjikakou, date: 07/20/2019, view: 622, Q&A: 0
The in vitro and in vivo genotoxicity of new metallodrugs either as Small Bioactive Molecules (SBAMs) or Conjugates of Metals with Drugs (CoMeDs) is evaluated by the micronucleus test and the Allium cepa assay, respectively. Fetal lung fibroblast cells (MRC-5), normal human corneal epithelial cells (HCEC) and ...
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Cell Biology

Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs Authors:  Lucy Ngo, Anton D. Nathanson, Tomasz Garbowski, Ulf Knothe, Dirk Zeidler and Melissa L. Knothe Tate, date: 07/20/2019, view: 464, Q&A: 0
Multibeam scanning electron microscopy (multiSEM) provides a technical platform for seamless nano-to-mesoscale mapping of cells in human tissues and organs, which is a major new initiative of the U.S. National Institutes of Health. Such cross-length-scale imaging is expected to provide unprecedented understanding of relationships between cellular ...
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Lipid-exchange Rate Assay for Lipid Droplet Fusion in Live Cells Authors:  Jia Wang, Boon Tin Chua, Peng Li and Feng-Jung Chen, date: 07/20/2019, view: 489, Q&A: 0
Lipid droplets (LDs) are central organelles in maintaining lipid homeostasis. Defective LD growth often results in the development of metabolic disorders. LD fusion and growth mediated by cell death–inducing DNA fragmentation factor alpha (DFFA)-like effector (CIDE) family proteins are crucial for various biological processes including unilocular ...
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Immunology

Isolation and Long-term Cultivation of Mouse Alveolar Macrophages Authors:  Clara Jana-Lui Busch, Jérémy Favret, Laufey Geirsdóttir, Kaaweh Molawi and Michael H. Sieweke, date: 07/20/2019, view: 807, Q&A: 0
Alveolar macrophages (AM) are tissue-resident macrophages that colonize the lung around birth and can self-maintain long-term in an adult organism without contribution of monocytes. AM are located in the pulmonary alveoli and can be harvested by washing the lungs using the method of bronchoalveolar lavage (BAL). Here, we compared different ...
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Microbiology

A Protocol to Map the Spatial Proteome Using HyperLOPIT in Saccharomyces cerevisiae Authors:  Daniel J. H. Nightingale, Kathryn S. Lilley and Stephen G. Oliver, date: 07/20/2019, view: 552, Q&A: 0
The correct subcellular localization of proteins is vital for cellular function and the study of this process at the systems level will therefore enrich our understanding of the roles of proteins within the cell. Multiple methods are available for the study of protein subcellular localization, including fluorescence microscopy, organelle ...
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Neuroscience

A Standardized Tank Design for the Light Dark Task in Zebrafish Authors:  Amanda Facciol, Steven Tran and Robert Gerlai, date: 07/20/2019, view: 309, Q&A: 0
The light dark paradigm is a common behavioral test used to screen a variety of pharmacological agents, including anxiogenics and anxiolytics. Although most often used in rodents, the light dark task has recently been adapted for use in zebrafish. However, a number of inconsistent findings have been reported for this species. Some have found ...
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Protocol for Measuring Compulsive-like Feeding Behavior in Mice Authors:  Marta Fructuoso, Jose Espinosa-Carrasco, Ionas Erb, Cedric Notredame and Mara Dierssen, date: 07/20/2019, view: 421, Q&A: 0
Obesity is an important health problem with a strong environmental component that is acquiring pandemic proportion. The high availability of caloric dense foods promotes overeating potentially causing obesity. Animal models are key to validate novel therapeutic strategies, but researchers must carefully select the appropriate model to draw the ...
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Axon-seq for in Depth Analysis of the RNA Content of Neuronal Processes Authors:  Jik Nijssen, Julio Aguila and Eva Hedlund, date: 07/20/2019, view: 636, Q&A: 0
Neuronal processes have an RNA composition that is distinct from the cell body. Therefore, to fully understand neuronal biology in health and disease we need to study both somas, dendrites and axons. Here we describe a detailed protocol of a newly refined method, Axon-seq, for RNA sequencing of axons (and dendrites) grown in isolation using single ...
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Plant Science

Isolation of Powdery Mildew Haustoria from Infected Barley Authors:  Linhan Li, Benjamin Collier and Pietro D. Spanu, date: 07/20/2019, view: 598, Q&A: 0
Blumeria graminis is a fungus that causes powdery mildews on grasses, such as barley. Investigations of this pathogen present many challenges due to its obligate biotrophic nature. This means that the fungus can only grow in the presence of a living host plant. B. graminis forms epiphytic mycelia on the plant surface and feeding ...
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Quantification of Blumenol Derivatives as Leaf Biomarkers for Plant-AMF Association Authors:  Elisabeth Mindt, Ming Wang, Martin Schäfer, Rayko Halitschke and Ian T. Baldwin, date: 07/20/2019, view: 631, Q&A: 0
Symbiotic interactions between arbuscular mycorrhizal fungi (AMF) and plants are widespread among land plants and can be beneficial for both partners. The plant is provided with mineral nutrients such as nitrogen and phosphorous, whereas it provides carbon resources for the fungus in return. Due to the large economic and environmental impact, ...
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Stem Cell

Isolation and Culture of Single Myofiber and Immunostaining of Satellite Cells from Adult C57BL/6J Mice Authors:  Shujie Chen, Hongrong Ding, Xiangping Yao and Liwei Xie, date: 07/20/2019, view: 554, Q&A: 0
Myofiber isolation followed with ex vivo culture could recapitulate and visualize satellite cells (SCs) activation, proliferation, and differentiation. This approach could be taken to understand the physiology of satellite cells and the molecular mechanism of regulatory factors, in terms of the involvement of intrinsic factors over SCs ...
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