• Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Past Issues in 2019

Volume: 9 Issue: 2

Biochemistry

Click Chemistry (CuAAC) and Detection of Tagged de novo Synthesized Proteins in Drosophila Authors:  Kathrin Marter, Oliver Kobler, Ines Erdmann, Elaheh Soleimanpour, Peter Landgraf, Anke Müller, Julia Abele, Ulrich Thomas and Daniela C. Dieterich, date: 01/20/2019, view: 2373, Q&A: 0
Copper-catalyzed azide-alkyne-cycloaddition (CuAAC), also known as ‘click chemistry’ serves as a technique for bio-orthogonal, that is, bio-compatible labeling of macromolecules including proteins or lipids. Click chemistry has been widely used to covalently, selectively, and efficiently attach probes such as fluorophores or biotin to small ...
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Insulin Tolerance Test under Anaesthesia to Measure Tissue-specific Insulin-stimulated Glucose Disposal Authors:  Daniel J. Fazakerley, Andreas M. Fritzen, Marin E. Nelson, Ida H. Thorius, Kristen C. Cooke, Sean J. Humphrey, Gregory J. cooney and David E. James, date: 01/20/2019, view: 1791, Q&A: 0
Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic ...
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Cell Biology

A Method for Culturing Mouse Whisker Follicles to Study Circadian Rhythms ex vivo Authors:  Atsuhiro Nishida, Yoshiki Miyawaki, Koichi Node and Makoto Akashi, date: 01/20/2019, view: 1165, Q&A: 0
Ex vivo tissue-culture experiments are often performed in the field of circadian biology. The major aim of these experiments is to evaluate circadian characteristics such as period length at the tissue-autonomous level by monitoring clock gene expression in real time. This culture method is also used to examine the tissue specificity of ...
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Immunology

Assessment of Humoral Alloimmunity in Mixed Lymphocyte Reaction Authors:  Georgios Pissas and Theodoros Eleftheriadis, date: 01/20/2019, view: 1916, Q&A: 1
Humoral alloimmunity remains a significant and unresolved problem that constrains allograft survival. Thus, there is a need for the development of an easy, preferably non-radioactive, and inexpensive protocol for assessing the effect of various drug treatments on humoral alloimmunity. In order to satisfy this demand, we developed such a protocol ...
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Immunohistochemical Staining of TLR4 in Human Skeletal Muscle Samples Authors:  Daniela Verzola, Samantha Milanesi, Francesca Costigliolo and Giacomo Garibotto, date: 01/20/2019, view: 1315, Q&A: 0
Growing evidence suggests the involvement of TLR4, a receptor in the innate immune system, in muscle loss in uremia. Recently, we have evaluated TLR4 in human skeletal muscle from chronic kidney disease patients, by immunohistochemistry and image analysis. Unlike the commonly-used Western blot method, immunohistochemistry allows for the ...
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Microbiology

Intracellular Invasion and Killing Assay to Investigate the Effects of Binge Alcohol Toxicity in Murine Alveolar Macrophages Authors:  Victor Jimenez Jr and Fernando P Monroy, date: 01/20/2019, view: 1237, Q&A: 0
Alcohol consumption has diverse and well-documented effects on the human immune system and its ability to defend against infective agents. While pulmonary related infections can occur in healthy humans, binge alcohol use is recognized as a major health risk factor (Nelson et al., 1991). Although binge alcohol consumption has been ...
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Assessing Yeast Cell Survival Following Hydrogen Peroxide Exposure Authors:  Khoa Tran and Erin M. Green, date: 01/20/2019, view: 2288, Q&A: 0
In the presence of oxidative stress, cellular defense systems that can detoxify reactive oxygen species are activated through multiple signaling cascades and transcriptional reprogramming. The budding yeast Saccharomyces cerevisiae has served as an excellent model for genetically-identifying factors important for the response to oxidative ...
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Molecular Biology

CRISPR-Cas9 Mediated Genome Editing in Drosophila Authors:  Ping Peng, Xia Wang, Da Shen, Jin Sun, Yu Jia, Rong-Gang Xu, Li-Fei Zhu and Jian-Quan Ni , date: 01/20/2019, view: 2187, Q&A: 0
In recent years, great progress has been made in the research of genome editing systems, one of which is the CRISPR-Cas9 system, a powerful technology that is applied to edit animal genome. Here, we describe a CRISPR-Cas9 mediated mutation protocol for efficiently and specifically editing genes in Drosophila. In this optimized system, the ...
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flySAM Transgenic CRISPRa System Manual Authors:  Yu Jia, Da Shen, Xia Wang, Jin Sun, Ping Peng, Rong-Gang Xu, Bowen Xu and Jian-Quan Ni , date: 01/20/2019, view: 1868, Q&A: 0
Powerful and general methods that can enhance gene expression are useful to systematically study gene function. To date, compared with the methods in generating loss-of-function mutants, methods to achieve gain-of-function are limited. The entire field in Drosophila has relied heavily on the Gal4/UAS:cDNA overexpression system developed ...
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Neuroscience

A Mouse Model of Postoperative Pain Authors:  Ashley M. Cowie and Cheryl L. Stucky, date: 01/20/2019, view: 2341, Q&A: 0
Postoperative pain is highly debilitating and hinders recovery. Opioids are the main pain medication used for acute postoperative pain. Given the devastating opioid addiction and overdose epidemic across the US, non-opioid pain therapeutics are desperately needed. In order to develop novel, non-opioid therapies for the treatment of postoperative ...
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High-resolution Immunoelectron Microscopy Techniques for Revealing Distinct Subcellular Type 1 Cannabinoid Receptor Domains in Brain Authors:  Nagore Puente, Itziar Bonilla-Del Río, Svein Achicallende, Patrick C. Nahirney and Pedro Grandes, date: 01/20/2019, view: 1679, Q&A: 0
Activation of type 1 cannabinoid (CB1) receptors by endogenous, exogenous (cannabis derivatives) or synthetic cannabinoids (i.e., CP 55.940, Win-2) has a wide variety of behavioral effects due to the presence of CB1 receptors in the brain. In situ hybridization and immunohistochemical techniques have been ...
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