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Modification and Application of a Commercial Whole-body Plethysmograph to Monitor Respiratory Abnormalities in Neonatal Mice

Featured protocol,  Authors: Yen-Ting Lai
Yen-Ting LaiAffiliation: Institute of Biomedical Sciences, Academia Sinica, 11529, Taipei, Taiwan
Bio-protocol author page: a4679
 and Yi-Shuian Huang
Yi-Shuian HuangAffiliation: Institute of Biomedical Sciences, Academia Sinica, 11529, Taipei, Taiwan
For correspondence: yishuian@ibms.sinica.edu.tw
Bio-protocol author page: a4680
date: 6/20/2017, 82 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2343.

Brief version appeared in J Neurosci, Dec 2016
Proper breathing is essential for mammals to acquire oxygen after birth and requires coordinated actions among several tissues, including diaphragm, intercostal muscles, trachea, bronchi, lung and respiration-regulating neurons located in the medulla oblongata. Genetically modified mice that die early postnatally may have respiratory defects caused by maldevelopment of any one of these tissues (Turgeon and Meloche, 2009). Because of the small body size of neonatal pups, whole-body plethysmography can be used to monitor their respiratory activities. In this protocol, we modified the commercial whole-body plethysmograph by increasing metal filters in the pneumotach, connecting an extension tube to the pneumotach and removing the bias flow supply. With these modifications, the sensitivity of this device is significantly increased to enable the detection of rhythmic respiration in neonatal mice as early as postnatal day 1 (P1).

Modification and Application of a Commercial Whole-body Plethysmograph to Monitor Respiratory Abnormalities in Neonatal Mice

Authors: Yen-Ting Lai
Yen-Ting LaiAffiliation: Institute of Biomedical Sciences, Academia Sinica, 11529, Taipei, Taiwan
Bio-protocol author page: a4679
 and Yi-Shuian Huang
Yi-Shuian HuangAffiliation: Institute of Biomedical Sciences, Academia Sinica, 11529, Taipei, Taiwan
For correspondence: yishuian@ibms.sinica.edu.tw
Bio-protocol author page: a4680
date: 6/20/2017, 82 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2343.

[Abstract] Proper breathing is essential for mammals to acquire oxygen after birth and requires coordinated actions among several tissues, including diaphragm, intercostal muscles, trachea, bronchi, lung and respiration-regulating neurons located in the medulla oblongata. Genetically modified mice that die early postnatally may have respiratory defects caused ...
1 

Modification and Application of a Commercial Whole-body Plethysmograph to Monitor Respiratory Abnormalities in Neonatal Mice

Authors: Yen-Ting Lai
Yen-Ting LaiAffiliation: Institute of Biomedical Sciences, Academia Sinica, 11529, Taipei, Taiwan
Bio-protocol author page: a4679
 and Yi-Shuian Huang
Yi-Shuian HuangAffiliation: Institute of Biomedical Sciences, Academia Sinica, 11529, Taipei, Taiwan
For correspondence: yishuian@ibms.sinica.edu.tw
Bio-protocol author page: a4680
date: 6/20/2017, 82 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2343.

[Abstract] Proper breathing is essential for mammals to acquire oxygen after birth and requires coordinated actions among several tissues, including diaphragm, intercostal muscles, trachea, bronchi, lung and respiration-regulating neurons located in the medulla oblongata. Genetically modified mice that die early ...
1