Authors: Alberto Rissone
and Fabio Candotti Alberto RissoneAffiliation 1:
Translational and Functional Genomics Branch, National Human Research Institute (NHGRI), National Institutes of Health (NIH), Bethesda, MD, USAAffiliation 2:
Disorders of Immunity Section, Genetics and Molecular Biology Branch, NHGRI, NIH, Bethesda, MD, USAFor correspondence: email@example.comBio-protocol author page: a3533
date: 10/5/2016, 168 views, 0 Q&A.
Fabio CandottiAffiliation 1:
Disorders of Immunity Section, Genetics and Molecular Biology Branch, NHGRI, NIH, Bethesda, MD, USAAffiliation 2:
Division of Immunology and Allergy, University Hospital of Lausanne, Lausanne, SwitzerlandFor correspondence: Fabio.Candotti@chuv.chBio-protocol author page: a3534
|Brief version appeared in J Exp Med, Jul 2015 |
The production of free radicals is the result of normal cellular metabolism. Free radicals are involved in innumerable different cellular and biological functions such as signaling, proliferation, cell death, aging, inflammation, etc
. Under physiological conditions, the levels of reactive oxygen species (ROS) are strictly regulated by the cells. However, during stressful conditions such as oxidative stress, ROS levels increase causing damages to different molecules like DNA, lipids and proteins. Increased levels of ROS have been associated with a growing list of different diseases. In this protocol, we used MitoSOX and CellROX Green oxidative stress probes to label the intracellular ROS and detect the fluorescence using cell sorting and confocal analyses.