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Fat Turnover Assay in Drosophila

Author: Subhash D. Katewa
Subhash D. KatewaAffiliation: Buck Institute for Research on Aging, Novato, CA, USA
For correspondence: skatewa@buckinstitute.org
Bio-protocol author page: a3681
date: 11/5/2016, 212 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1996.

[Abstract] Like all animals, Drosophila shows robust fat (triglyceride) turnover, i.e., they synthesize, store and utilize triglyceride for their daily metabolic needs. The protocol describes a simple assay to measure this turnover of triglycerides in Drosophila.

[Background] Almost all animals store energy reserves ...

Protein Expression, Purification and Crystallization of the Sxl-Unr-msl2 Ribonucleoprotein Complex

Authors: Janosch Hennig
Janosch HennigAffiliation: Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL) Heidelberg, Germany
For correspondence: janosch.hennig@helmholtz-muenchen.de
Bio-protocol author page: a3470
 and Michael Sattler
Michael SattlerAffiliation 1: Center for Integrated Protein Science Munich (CIPSM) at Department of Chemistry, Technische UniversitätMünchen, Germany
Affiliation 2: Institute of Structural Biology, Helmholtz ZentrumMünchen, Germany
For correspondence: sattler@helmholtz-muenchen.de
Bio-protocol author page: a3471
date: 9/5/2016, 420 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1917.

[Abstract] This protocol describes the expression, purification and crystallization of a ternary protein-protein-RNA complex, consisting of the two RNA recognition motifs (RRMs) of Sex-lethal (Sxl), the first of five cold shock domains of Upstream-of-N-Ras (Unr), and an 18-nucleotide region of msl2 mRNA, called ...

Bioassays to Investigate the Effects of Insect Oviposition on a Plant’s Resistance to Herbivores

Authors: Michele Bandoly
Michele BandolyAffiliation: Institute of Biology, Dahlem Centre of Plant Sciences (DCPS), Molecular Ecology, Freie Universität (FU) Berlin, Berlin, Germany
Bio-protocol author page: a3162
 and Anke Steppuhn
Anke SteppuhnAffiliation: Institute of Biology, Dahlem Centre of Plant Sciences (DCPS), Molecular Ecology, Freie Universität (FU) Berlin, Berlin, Germany
For correspondence: a.steppuhn@fu-berlin.de
Bio-protocol author page: a3163
date: 6/5/2016, 852 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1823.

[Abstract] Plants respond to herbivory with diverse defence responses (Schoonhoven et al., 2005). Many herbivorous insects deposit their eggs on their host plants before their larvae start to feed. Thus, plants could use insect eggs as a signal to increase their resistance to herbivores. Here, we report experimental ...

Preparation and Immunofluorescence Staining of the Trachea in Drosophila Larvae and Pupae

Author: Feng Chen
Feng ChenAffiliation: Department of Biochemistry, Stanford University School of Medicine, Stanford, USA
For correspondence: fchen66@alumni.stanford.edu
Bio-protocol author page: a3101
date: 5/5/2016, 1102 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1797.

[Abstract] The Drosophila melanogaster trachea is a branched network of rigid chitin-lined tubes that ramify throughout the body and functions as the fly’s respiratory organ. Small openings at the ends of the tracheal tubes allow gas exchange to occur by diffusion between internal tissues and the exterior environment. ...

Electroretinogram (ERG) Recordings from Drosophila

Authors: Elisheva Rhodes-Mordov*
Elisheva Rhodes-MordovAffiliation: Departments of Medical Neurobiology, the Institute of Medical Research Israel-Canada (IMRIC) and the Edmond and Lily Safra Center for Brain Sciences (ELSC), Faculty of Medicine of the Hebrew University, Jerusalem, Israel
Bio-protocol author page: a2672
Hadar Samra*
Hadar SamraAffiliation: Departments of Medical Neurobiology, the Institute of Medical Research Israel-Canada (IMRIC) and the Edmond and Lily Safra Center for Brain Sciences (ELSC), Faculty of Medicine of the Hebrew University, Jerusalem, Israel
Bio-protocol author page: a2673
 and Baruch Minke
Baruch MinkeAffiliation: Departments of Medical Neurobiology, the Institute of Medical Research Israel-Canada (IMRIC) and the Edmond and Lily Safra Center for Brain Sciences (ELSC), Faculty of Medicine of the Hebrew University, Jerusalem, Israel
For correspondence: baruchm@ekmd.huji.ac.il
Bio-protocol author page: a2674
 (*contributed equally to this work) date: 11/5/2015, 1455 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1636.

[Abstract] Phototransduction is a process in which light is converted into electrical signals used by the central nervous system. Invertebrate phototransduction is a process mediated by the phosphoinositide signaling cascade, characterized by Phospholipase C (PLC) as the effector enzyme and the Transient Receptor ...

FLP/FRT Induction of Mitotic Recombination in Drosophila Germline

Authors: Pedro Prudêncio
Pedro PrudêncioAffiliation 1: Regenerative Medicine Program, Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 2: IBB-Institute for Biotechnology and Bioengineering, CBME-Centro de Biomedicina Molecular e Estrutural, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 3: Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, Oeiras, Portugal
Bio-protocol author page: a2168
 and Leonardo G. Guilgur
Leonardo G. GuilgurAffiliation 1: Regenerative Medicine Program, Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 2: IBB-Institute for Biotechnology and Bioengineering, CBME-Centro de Biomedicina Molecular e Estrutural, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 3: Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, Oeiras, Portugal
For correspondence: lguilgur@igc.gulbenkian.pt
Bio-protocol author page: a2169
date: 5/5/2015, 4261 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1458.

[Abstract] The FLP/FRT system is a site-directed recombination technology based on the targeting of a recombination enzyme (flipase - FLP) to specific DNA regions designated as flipase recognition target (FRT) sites. Initially identified in Saccharomyces cerevisiae, the yeast FLP-enzyme and its FRT recombination ...

Protein Extraction from Drosophila Embryos and Ovaries

Authors: Pedro Prudêncio
Pedro PrudêncioAffiliation 1: Regenerative Medicine Program, Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 2: IBB-Institute for Biotechnology and Bioengineering, CBME-Centro de Biomedicina Molecular e Estrutural, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 3: Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, Oeiras, Portugal
Bio-protocol author page: a2168
 and Leonardo G. Guilgur
Leonardo G. GuilgurAffiliation 1: Regenerative Medicine Program, Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 2: IBB-Institute for Biotechnology and Bioengineering, CBME-Centro de Biomedicina Molecular e Estrutural, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 3: Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, Oeiras, Portugal
For correspondence: lguilgur@igc.gulbenkian.pt
Bio-protocol author page: a2169
date: 5/5/2015, 3284 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1459.

[Abstract] Here we provide the description of protocols to efficiently obtain protein extracts from embryos and ovaries of Drosophila melanogaster. These protocols are routinely applied in our laboratory and are based on two techniques: either embryos or ovaries are homogenized using a pestle and then the soluble ...

Measuring Anxiety-like Behavior in Crayfish by Using a Sub Aquatic Dark-light Plus Maze

Authors: Pascal Fossat
Pascal FossatAffiliation: Université de Bordeaux, Bordeaux, France
For correspondence: pascal.fossat@u-bordeaux.fr
Bio-protocol author page: a1985
Julien Bacqué-Cazenave
Julien Bacqué-CazenaveAffiliation: Université de Bordeaux, Bordeaux, France
Bio-protocol author page: a1986
Jean-Paul Delbecque*
Jean-Paul DelbecqueAffiliation 1: Université de Bordeaux, Bordeaux, France
Affiliation 2: Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences Cognitives et Intégratives d’Aquitaine, Avenue des Facultés, Talence Cedex, France
Bio-protocol author page: a1987
 and Daniel Cattaert*
Daniel CattaertAffiliation 1: Université de Bordeaux, Bordeaux, France
Affiliation 2: Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences Cognitives et Intégratives d’Aquitaine, Avenue des Facultés, Talence Cedex, France
Bio-protocol author page: a1988
 (*contributed equally to this work) date: 2/5/2015, 2179 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1396.

[Abstract] Crayfish are omnivorous freshwater arthropods that naturally explore their environment during day and night, but also frequently hide under a shelter or in a hole in case of danger. They may be submitted to various stressors, including predation, social interactions or changes in environmental parameters ...

Electrical Penetration Graph Recording (Whitefly)

Authors: Baiming Liu
Baiming LiuAffiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China
Bio-protocol author page: a1202
Xuguo Zhou
Xuguo ZhouAffiliation 1: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China
Affiliation 2: Department of Entomology, University of Kentucky, Lexington, USA
For correspondence: xuguozhou@uky.edu
Bio-protocol author page: a1203
 and Youjun Zhang
Youjun ZhangAffiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China
For correspondence: zhangyoujun@caas.cn
Bio-protocol author page: a1204
date: 3/5/2014, 3300 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1063.

[Abstract] Electrical penetration graph (EPG) was first developed by Mclean and Kinsey (1964) and later modified by Tjallingii (1978 and 1985). The EPG system turns a phloem-sucking insects and its host plant into part of an electrical circuit that is completed when the insect’s mouthparts penetrate the plant. ...

Fluorescence in situ Hybridization to the Polytene Chromosomes of Anopheles Mosquitoes

Authors: Ai Xia
Ai XiaAffiliation: Department of Entomology, Nanjing Agricultural University, Nanjing, China
Bio-protocol author page: a757
Ashley Peery
Ashley PeeryAffiliation: Department of Entomology, Virginia Tech, Blacksburg, USA
Bio-protocol author page: a758
Maryam Kamali
Maryam KamaliAffiliation: Department of Entomology, Virginia Tech, Blacksburg, USA
Bio-protocol author page: a759
Jiangtao Liang
Jiangtao LiangAffiliation: Department of Entomology, Nanjing Agricultural University, Nanjing, China
Bio-protocol author page: a760
Maria V. Sharakhova
Maria V. SharakhovaAffiliation: Department of Entomology, Virginia Tech, Blacksburg, USA
Bio-protocol author page: a761
 and Igor V. Sharakhov
Igor V. SharakhovAffiliation: Department of Entomology, Virginia Tech, Blacksburg, USA
For correspondence: igor@vt.edu
Bio-protocol author page: a427
date: 8/20/2013, 3829 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.860.

[Abstract] Fluorescence in situ hybridization (FISH) is a method that uses a fluorescently labeled DNA probe for mapping the position of a genetic element on chromosomes. A DNA probe is prepared by incorporating Cy-3 or Cy-5 labeled nucleotides into DNA by nick-translation or a random primed labeling method. This ...
1 2 

[Bio101] Transfection of S2 Cell with DNA Using CellFectin Reagent

Author: Darran G. Cronshaw
Darran G. CronshawAffiliation: Center for Autoimmune and Musculoskeletal Diseases, Feinstein Institute for Medical Research, Manhasset, USA
For correspondence:
Bio-protocol author page: a41
date: 3/5/2012, 9584 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.190.

[Abstract] This method provides a step-by-step guide to transfecting Drosophila S2 cells with the pRmHA-3 (or similar) vector with insert of choice (in this case SDF-1β-FLAG) and generating a stable cell line. This cell line is then capable of producing the protein of interest under inducible conditions by addition ...

Tandem Affinity Purification in Drosophila Heads and Ovaries

Authors: Anita Pepper
Anita PepperAffiliation: Department of Genetics, The Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
Bio-protocol author page: a75
Balpreet Bhogal
Balpreet BhogalAffiliation: Department of Genetics, The Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
Bio-protocol author page: a76
 and Thomas Jongens
Thomas JongensAffiliation: Department of Genetics, The Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
For correspondence: jongens@mail.med.upenn.edu
Bio-protocol author page: a77
date: 8/5/2012, 5909 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.245.

[Abstract] Tandem affinity purification (TAP) (Pugi et al.,2001; Rigaut et al., 1999) is a method that uses a tagging approach of a target protein of interest for a two-step purification scheme in order to pull down protein complexes under native conditions and expression levels. The TAP tag consists of three ...

FLP/FRT Induction of Mitotic Recombination in Drosophila Germline

Authors: Pedro Prudêncio
Pedro PrudêncioAffiliation 1: Regenerative Medicine Program, Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 2: IBB-Institute for Biotechnology and Bioengineering, CBME-Centro de Biomedicina Molecular e Estrutural, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 3: Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, Oeiras, Portugal
Bio-protocol author page: a2168
 and Leonardo G. Guilgur
Leonardo G. GuilgurAffiliation 1: Regenerative Medicine Program, Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 2: IBB-Institute for Biotechnology and Bioengineering, CBME-Centro de Biomedicina Molecular e Estrutural, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 3: Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, Oeiras, Portugal
For correspondence: lguilgur@igc.gulbenkian.pt
Bio-protocol author page: a2169
date: 5/5/2015, 4261 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1458.

[Abstract] The FLP/FRT system is a site-directed recombination technology based on the targeting of a recombination enzyme (flipase - FLP) to specific DNA regions designated as flipase recognition target (FRT) sites. Initially identified in Saccharomyces cerevisiae, the yeast FLP-enzyme and its FRT recombination ...

Fluorescence in situ Hybridization to the Polytene Chromosomes of Anopheles Mosquitoes

Authors: Ai Xia
Ai XiaAffiliation: Department of Entomology, Nanjing Agricultural University, Nanjing, China
Bio-protocol author page: a757
Ashley Peery
Ashley PeeryAffiliation: Department of Entomology, Virginia Tech, Blacksburg, USA
Bio-protocol author page: a758
Maryam Kamali
Maryam KamaliAffiliation: Department of Entomology, Virginia Tech, Blacksburg, USA
Bio-protocol author page: a759
Jiangtao Liang
Jiangtao LiangAffiliation: Department of Entomology, Nanjing Agricultural University, Nanjing, China
Bio-protocol author page: a760
Maria V. Sharakhova
Maria V. SharakhovaAffiliation: Department of Entomology, Virginia Tech, Blacksburg, USA
Bio-protocol author page: a761
 and Igor V. Sharakhov
Igor V. SharakhovAffiliation: Department of Entomology, Virginia Tech, Blacksburg, USA
For correspondence: igor@vt.edu
Bio-protocol author page: a427
date: 8/20/2013, 3829 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.860.

[Abstract] Fluorescence in situ hybridization (FISH) is a method that uses a fluorescently labeled DNA probe for mapping the position of a genetic element on chromosomes. A DNA probe is prepared by incorporating Cy-3 or Cy-5 labeled nucleotides into DNA by nick-translation or a random primed labeling method. This ...

Electrical Penetration Graph Recording (Whitefly)

Authors: Baiming Liu
Baiming LiuAffiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China
Bio-protocol author page: a1202
Xuguo Zhou
Xuguo ZhouAffiliation 1: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China
Affiliation 2: Department of Entomology, University of Kentucky, Lexington, USA
For correspondence: xuguozhou@uky.edu
Bio-protocol author page: a1203
 and Youjun Zhang
Youjun ZhangAffiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China
For correspondence: zhangyoujun@caas.cn
Bio-protocol author page: a1204
date: 3/5/2014, 3300 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1063.

[Abstract] Electrical penetration graph (EPG) was first developed by Mclean and Kinsey (1964) and later modified by Tjallingii (1978 and 1985). The EPG system turns a phloem-sucking insects and its host plant into part of an electrical circuit that is completed when the insect’s mouthparts penetrate the plant. ...

Protein Extraction from Drosophila Embryos and Ovaries

Authors: Pedro Prudêncio
Pedro PrudêncioAffiliation 1: Regenerative Medicine Program, Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 2: IBB-Institute for Biotechnology and Bioengineering, CBME-Centro de Biomedicina Molecular e Estrutural, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 3: Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, Oeiras, Portugal
Bio-protocol author page: a2168
 and Leonardo G. Guilgur
Leonardo G. GuilgurAffiliation 1: Regenerative Medicine Program, Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 2: IBB-Institute for Biotechnology and Bioengineering, CBME-Centro de Biomedicina Molecular e Estrutural, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
Affiliation 3: Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, Oeiras, Portugal
For correspondence: lguilgur@igc.gulbenkian.pt
Bio-protocol author page: a2169
date: 5/5/2015, 3284 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1459.

[Abstract] Here we provide the description of protocols to efficiently obtain protein extracts from embryos and ovaries of Drosophila melanogaster. These protocols are routinely applied in our laboratory and are based on two techniques: either embryos or ovaries are homogenized using a pestle and then the soluble ...

LC3B Labeling on Terrestrial Isopod Adipocytes

Authors: Christine Braquart-Varnier
Christine Braquart-VarnierAffiliation: Ecologie et Biologie des Interactions, UMR 7267 CNRS, Université de Poitiers, Poitiers Cedex, France
For correspondence: christine.braquart@univ-poitiers.fr
Bio-protocol author page: a625
Maryline Raimond
Maryline RaimondAffiliation: Ecologie et Biologie des Interactions, UMR 7267 CNRS, Université de Poitiers, Poitiers Cedex, France
Bio-protocol author page: a626
 and Mathieu Sicard
Mathieu SicardAffiliation: Ecologie et Biologie des Interactions, UMR 7267 CNRS, Université de Poitiers, Poitiers Cedex, France
Bio-protocol author page: a341
date: 6/20/2013, 3176 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.792.

[Abstract] The LC3B protein plays a critical role in autophagy. Normally, this protein resides in the cytosol, but following cleavage and lipidation with phosphatidylethanolamine, LC3B associates with the phagophore. This localization can be used as a general marker for autophagic membranes. To visualize the LC3B, ...

Measuring Anxiety-like Behavior in Crayfish by Using a Sub Aquatic Dark-light Plus Maze

Authors: Pascal Fossat
Pascal FossatAffiliation: Université de Bordeaux, Bordeaux, France
For correspondence: pascal.fossat@u-bordeaux.fr
Bio-protocol author page: a1985
Julien Bacqué-Cazenave
Julien Bacqué-CazenaveAffiliation: Université de Bordeaux, Bordeaux, France
Bio-protocol author page: a1986
Jean-Paul Delbecque*
Jean-Paul DelbecqueAffiliation 1: Université de Bordeaux, Bordeaux, France
Affiliation 2: Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences Cognitives et Intégratives d’Aquitaine, Avenue des Facultés, Talence Cedex, France
Bio-protocol author page: a1987
 and Daniel Cattaert*
Daniel CattaertAffiliation 1: Université de Bordeaux, Bordeaux, France
Affiliation 2: Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences Cognitives et Intégratives d’Aquitaine, Avenue des Facultés, Talence Cedex, France
Bio-protocol author page: a1988
 (*contributed equally to this work) date: 2/5/2015, 2179 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1396.

[Abstract] Crayfish are omnivorous freshwater arthropods that naturally explore their environment during day and night, but also frequently hide under a shelter or in a hole in case of danger. They may be submitted to various stressors, including predation, social interactions or changes in environmental parameters ...

Electroretinogram (ERG) Recordings from Drosophila

Authors: Elisheva Rhodes-Mordov*
Elisheva Rhodes-MordovAffiliation: Departments of Medical Neurobiology, the Institute of Medical Research Israel-Canada (IMRIC) and the Edmond and Lily Safra Center for Brain Sciences (ELSC), Faculty of Medicine of the Hebrew University, Jerusalem, Israel
Bio-protocol author page: a2672
Hadar Samra*
Hadar SamraAffiliation: Departments of Medical Neurobiology, the Institute of Medical Research Israel-Canada (IMRIC) and the Edmond and Lily Safra Center for Brain Sciences (ELSC), Faculty of Medicine of the Hebrew University, Jerusalem, Israel
Bio-protocol author page: a2673
 and Baruch Minke
Baruch MinkeAffiliation: Departments of Medical Neurobiology, the Institute of Medical Research Israel-Canada (IMRIC) and the Edmond and Lily Safra Center for Brain Sciences (ELSC), Faculty of Medicine of the Hebrew University, Jerusalem, Israel
For correspondence: baruchm@ekmd.huji.ac.il
Bio-protocol author page: a2674
 (*contributed equally to this work) date: 11/5/2015, 1455 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1636.

[Abstract] Phototransduction is a process in which light is converted into electrical signals used by the central nervous system. Invertebrate phototransduction is a process mediated by the phosphoinositide signaling cascade, characterized by Phospholipase C (PLC) as the effector enzyme and the Transient Receptor ...

Preparation and Immunofluorescence Staining of the Trachea in Drosophila Larvae and Pupae

Author: Feng Chen
Feng ChenAffiliation: Department of Biochemistry, Stanford University School of Medicine, Stanford, USA
For correspondence: fchen66@alumni.stanford.edu
Bio-protocol author page: a3101
date: 5/5/2016, 1102 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1797.

[Abstract] The Drosophila melanogaster trachea is a branched network of rigid chitin-lined tubes that ramify throughout the body and functions as the fly’s respiratory organ. Small openings at the ends of the tracheal tubes allow gas exchange to occur by diffusion between internal tissues and the exterior environment. ...
1 2