Authors: Ithai Rabinowitch
, Millet Treinin Ithai RabinowitchAffiliation:
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, USAFor correspondence: email@example.comBio-protocol author page: a3605
and Jihong Bai Millet TreininAffiliation:
Department of Medical Neurobiology, Hadassah Medical School, Hebrew University of Jerusalem, Jerusalem, IsraelBio-protocol author page: a3606
date: 10/20/2016, 22 views, 0 Q&A.
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, USABio-protocol author page: a3607
|Brief version appeared in PLoS Biol, Jan 2016 |
Optogenetics is a powerful tool for manipulating neuronal activity with high temporal and spatial precision. In the nematode C. elegans
optogentics is especially useful and easy to apply. This is because C. elegans
is translucent, so its neurons are highly accessible to optic stimulation. In addition, many of its neurons can be exclusively targeted using cell-specific promoters. We have recently taken advantage of optogentics to deliver artificial patterns of prolonged activation to a class of mechanosensory neurons, called touch receptor neurons (TRNs) in worms that lack touch sensation due to a genetic mutation. Our aim was to examine whether we can counteract the effects of sensory loss by artificially activating the sensory neurons. Here we describe in detail the various components of the protocol that we used. This consists of exposing worms expressing the light-sensitive ion channel Channelrohdopsin 2 (ChR2) in TRNs to long-term random flashes of light.
Authors: Pietro Tedesco
, Elia Di Schiavi Pietro TedescoAffiliation:
Institute of Protein Biochemistry, National Research Council, Naples, ItalyBio-protocol author page: a3599
, Fortunato Palma Esposito Elia Di SchiaviAffiliation:
Institute of Bioscience and Bioresources, National Research Council, Naples, ItalyBio-protocol author page: a3600
and Donatella de Pascale Fortunato Palma EspositoAffiliation:
Institute of Protein Biochemistry, National Research Council, Naples, ItalyBio-protocol author page: a3601
date: 10/20/2016, 25 views, 0 Q&A.
Donatella de PascaleAffiliation:
Institute of Protein Biochemistry, National Research Council, Naples, ItalyFor correspondence: firstname.lastname@example.orgBio-protocol author page: a3602
|Brief version appeared in PlOS ONE, Nov 2015 |
This protocol describes two biological assays to evaluate pathogenicity of Burkholderia cepacia
complex (Bcc) strains against the nematode Caenorhabditis elegans
. Specifically, these two assays allow one to identify if the under-investigated Bcc strains are able to kill the nematodes by intestinal colonization (slow killing assay, SKA) or by toxins production (fast killing assay, FKA). The principal differences between the two assays rely on the different killing kinetics for worms.