Welcome guest, Sign in

Home

Worms

Imaging the Pharynx to Measure the Uptake of Doxorubicin in Caenorhabditis elegans

Featured protocol,  Authors: Sivathevy Amirthagunabalasingam
Sivathevy AmirthagunabalasingamAffiliation: Maisonneuve-Rosemont Hospital Research Center, and the Université de Montréal, Faculty of Medicine, Department of Medicine, Montréal, Canada
Bio-protocol author page: a4534
Arturo Papaluca
Arturo PapalucaAffiliation: , Lady Davis Institute, McGill University, Montréal, Canada
Bio-protocol author page: a4535
Taramatti Harihar
Taramatti HariharAffiliation: Maisonneuve-Rosemont Hospital Research Center, and the Université de Montréal, Faculty of Medicine, Department of Medicine, Montréal, Canada
Bio-protocol author page: a4536
 and Dindial Ramotar
Dindial RamotarAffiliation: Maisonneuve-Rosemont Hospital Research Center, and the Université de Montréal, Faculty of Medicine, Department of Medicine, Montréal, Canada
For correspondence: dindial.ramotar@umontreal.ca
Bio-protocol author page: a4537
date: 5/20/2017, 98 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2291.

Brief version appeared in Sci Rep, Oct 2016
Caenorhabditis elegans offers an array of advantages to investigate the roles of uptake transporters. Herein, an epifluorescent microscopy approach was developed to monitor the uptake of the autofluorescent anticancer drug, doxorubicin, into the pharynx of C. elegans by organic cation transporters.

Nuclei Isolation from Nematode Ascaris

Featured protocol,  Authors: Yuanyuan Kang
Yuanyuan KangAffiliation: Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, USA
Bio-protocol author page: a4463
Jianbin Wang
Jianbin WangAffiliation: Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, USA
Bio-protocol author page: a4464
 and Richard E. Davis
Richard E. DavisAffiliation: Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, USA
For correspondence: richard.davis@ucdenver.edu
Bio-protocol author page: a4465
date: 5/5/2017, 185 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2262.

Brief version appeared in Cell Rep, Aug 2016
Preparing nuclei is necessary in a variety of experimental paradigms to study nuclear processes. In this protocol, we describe a method for rapid preparation of large number of relatively pure nuclei from Ascaris embryos or tissues that are ready to be used for further experiments such as chromatin isolation and ChIP-seq, nuclear RNA analyses, or preparation of nuclear extracts (Kang et al., 2016; Wang et al., 2016).

Electroshock Induced Seizures in Adult C. elegans

Featured protocol,  Authors: Monica G Risley
Monica G RisleyAffiliation 1: Department of Biological Sciences, Florida Atlantic University, Boca Raton, USA
Affiliation 2: International Max Planck Research School (IMPRS) for Brain and Behavior, Boca Raton, USA
Bio-protocol author page: a4483
Stephanie P Kelly
Stephanie P KellyAffiliation: Department of Biological Sciences, Florida Atlantic University, Boca Raton, United States
Bio-protocol author page: a4484
 and Ken Dawson-Scully
Ken Dawson-ScullyAffiliation: Department of Biological Sciences, Florida Atlantic University, Boca Raton, USA
For correspondence: ken.dawson-scully@fau.edu
Bio-protocol author page: a4485
date: 5/5/2017, 163 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2270.

Brief version appeared in PLoS One, Sep 2016
The nematode Caenorhabditis elegans is a useful model organism for dissecting molecular mechanisms of neurological diseases. While hermaphrodite C. elegans contain only 302 neurons, the conserved homologous neurotransmitters, simpler neuronal circuitry, and fully mapped connectome make it an appealing model system for neurological research. Here we developed an assay to induce an electroconvulsive seizure in C. elegans which can be used as a behavioral method of analyzing potential anti-epileptic therapeutics and novel genes involved in seizure susceptibility. In this assay, worms are suspended in an aqueous solution as current is passed through the liquid. At the onset of the shock, worms will briefly paralyze and twitch, and shortly after regain normal sinusoidal locomotion. The time to locomotor recovery is used as a metric of recovery from a seizure which can be reduced or extended by incorporating drugs that alter neuronal and muscular excitability.

Imaging the Pharynx to Measure the Uptake of Doxorubicin in Caenorhabditis elegans

Authors: Sivathevy Amirthagunabalasingam
Sivathevy AmirthagunabalasingamAffiliation: Maisonneuve-Rosemont Hospital Research Center, and the Université de Montréal, Faculty of Medicine, Department of Medicine, Montréal, Canada
Bio-protocol author page: a4534
Arturo Papaluca
Arturo PapalucaAffiliation: , Lady Davis Institute, McGill University, Montréal, Canada
Bio-protocol author page: a4535
Taramatti Harihar
Taramatti HariharAffiliation: Maisonneuve-Rosemont Hospital Research Center, and the Université de Montréal, Faculty of Medicine, Department of Medicine, Montréal, Canada
Bio-protocol author page: a4536
 and Dindial Ramotar
Dindial RamotarAffiliation: Maisonneuve-Rosemont Hospital Research Center, and the Université de Montréal, Faculty of Medicine, Department of Medicine, Montréal, Canada
For correspondence: dindial.ramotar@umontreal.ca
Bio-protocol author page: a4537
date: 5/20/2017, 98 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2291.

[Abstract] Caenorhabditis elegans offers an array of advantages to investigate the roles of uptake transporters. Herein, an epifluorescent microscopy approach was developed to monitor the uptake of the autofluorescent anticancer drug, doxorubicin, into the pharynx of C. elegans by organic cation transporters....

Nuclei Isolation from Nematode Ascaris

Authors: Yuanyuan Kang
Yuanyuan KangAffiliation: Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, USA
Bio-protocol author page: a4463
Jianbin Wang
Jianbin WangAffiliation: Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, USA
Bio-protocol author page: a4464
 and Richard E. Davis
Richard E. DavisAffiliation: Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, USA
For correspondence: richard.davis@ucdenver.edu
Bio-protocol author page: a4465
date: 5/5/2017, 185 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2262.

[Abstract] Preparing nuclei is necessary in a variety of experimental paradigms to study nuclear processes. In this protocol, we describe a method for rapid preparation of large number of relatively pure nuclei from Ascaris embryos or tissues that are ready to be used for further experiments such as chromatin ...

Electroshock Induced Seizures in Adult C. elegans

Authors: Monica G Risley
Monica G RisleyAffiliation 1: Department of Biological Sciences, Florida Atlantic University, Boca Raton, USA
Affiliation 2: International Max Planck Research School (IMPRS) for Brain and Behavior, Boca Raton, USA
Bio-protocol author page: a4483
Stephanie P Kelly
Stephanie P KellyAffiliation: Department of Biological Sciences, Florida Atlantic University, Boca Raton, United States
Bio-protocol author page: a4484
 and Ken Dawson-Scully
Ken Dawson-ScullyAffiliation: Department of Biological Sciences, Florida Atlantic University, Boca Raton, USA
For correspondence: ken.dawson-scully@fau.edu
Bio-protocol author page: a4485
date: 5/5/2017, 163 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2270.

[Abstract] The nematode Caenorhabditis elegans is a useful model organism for dissecting molecular mechanisms of neurological diseases. While hermaphrodite C. elegans contain only 302 neurons, the conserved homologous neurotransmitters, simpler neuronal circuitry, and fully mapped connectome make it an appealing ...

In vivo Mitophagy Monitoring in Caenorhabditis elegans to Determine Mitochondrial Homeostasis

Authors: Konstantinos Palikaras
Konstantinos PalikarasAffiliation: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece
Bio-protocol author page: a3832
 and Nektarios Tavernarakis
Nektarios TavernarakisAffiliation 1: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece
Affiliation 2: Department of Basic Sciences, Faculty of Medicine, University of Crete, Crete, Greece
For correspondence: tavernarakis@imbb.forth.gr
Bio-protocol author page: a3833
date: 4/5/2017, 294 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2215.

[Abstract] Perturbation of mitochondrial function is a major hallmark of several pathological conditions and ageing, underlining the essential role of fine-tuned mitochondrial activity (Lopez-Otin et al., 2013). Mitochondrial selective autophagy, known as mitophagy, mediates the removal of dysfunctional and/or ...

In vivo Live Imaging of Calcium Waves and Other Cellular Processes during Fertilization in Caenorhabditis elegans

Authors: Jun Takayama
Jun TakayamaAffiliation: RIKEN Quantitative Biology Center, Laboratory for Developmental Dynamics, Kobe, Japan
Bio-protocol author page: a4310
Masashi Fujita
Masashi FujitaAffiliation: RIKEN Quantitative Biology Center, Laboratory for Developmental Dynamics, Kobe, Japan
Bio-protocol author page: a4311
 and Shuichi Onami
Shuichi OnamiAffiliation: RIKEN Quantitative Biology Center, Laboratory for Developmental Dynamics, Kobe, Japan
For correspondence: sonami@riken.jp
Bio-protocol author page: a4312
date: 4/5/2017, 416 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2205.

[Abstract] Fertilization calcium waves are a conserved trigger for animal development; however, genetic analysis of these waves has been limited due to the difficulty of imaging in vivo fertilization. Here we describe a protocol to image calcium dynamics during in vivo fertilization in the genetic animal model ...

Measuring Caenorhabditis elegans Sleep during the Transition to Adulthood Using a Microfluidics-based System

Authors: Huiyan Huang
Huiyan HuangAffiliation: Department of Neuroscience, Brown University, Providence, USA
Bio-protocol author page: a4251
Komudi Singh
Komudi SinghAffiliation: Department of Neuroscience, Brown University, Providence, USA
Present address: Laboratory of Mitochondrial Biology & Metabolism, National Institute of Health, Bethesda, USA
Bio-protocol author page: a4252
 and Anne C. Hart
Anne C. HartAffiliation: Department of Neuroscience, Brown University, Providence, USA
For correspondence: anne_hart@brown.edu
Bio-protocol author page: a4253
date: 3/20/2017, 404 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2174.

[Abstract] C. elegans sleep during development is regulated by genes and cellular mechanisms that are conserved across the animal kingdom (Singh et al., 2014; Trojanowski and Raizen, 2016). C. elegans developmental sleep is usually assessed during the transition to adulthood, a 2.6 h time interval called lethargus ...

Protein Synthesis Rate Assessment by Fluorescence Recovery after Photobleaching (FRAP)

Authors: Nikos Kourtis
Nikos KourtisAffiliation 1: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece
Affiliation 2: Department of Pathology, NYU School of Medicine, New York, USA
Bio-protocol author page: a4141
 and Nektarios Tavernarakis
Nektarios TavernarakisAffiliation 1: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece
Affiliation 2: Department of Basic Sciences, Faculty of Medicine, University of Crete, Crete, Greece
For correspondence: tavernarakis@imbb.forth.gr
Bio-protocol author page: a3833
date: 3/5/2017, 571 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2156.

[Abstract] Currently available biochemical methods cannot be applied to monitor protein synthesis in specific cells or tissues, in live specimens. Here, we describe a non-invasive method for monitoring protein synthesis in single cells or tissues with intrinsically different translation rates, in live Caenorhabditis ...

Polyethylene Glycol-mediated Transformation of Drechmeria coniospora

Authors: Le D. He
Le D. HeAffiliation: Aix Marseille Univ, CNRS, INSERM, CIML, Centre d’Immunologie de Marseille-Luminy, Marseille, France
Bio-protocol author page: a4195
 and Jonathan J. Ewbank
Jonathan J. EwbankAffiliation: Aix Marseille Univ, CNRS, INSERM, CIML, Centre d’Immunologie de Marseille-Luminy, Marseille, France
For correspondence: ewbank@ciml.univ-mrs.fr
Bio-protocol author page: a4196
date: 3/5/2017, 518 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2157.

[Abstract] Drechmeria coniospora is a nematophagous fungus and potential biocontrol agent. It belongs to the Ascomycota. It is related to Hirsutella minnesotensis, another nematophagous fungus but, phylogenetically, it is currently closest to the truffle parasite Tolypocladium ophioglossoides. Together with its ...

P-body and Stress Granule Quantification in Caenorhabditis elegans

Authors: Matthias Rieckher
Matthias RieckherAffiliation: Institute for Genome Stability in Ageing and Disease, Cologne Cluster of Excellence in Cellular Stress Responses in Aging-associated Diseases (CECAD) Research Center, Center for Molecular Medicine (CMMC), University of Cologne, Cologne, Germany
Bio-protocol author page: a4014
 and Nektarios Tavernarakis
Nektarios TavernarakisAffiliation 1: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece
Affiliation 2: Department of Basic Sciences, Faculty of Medicine, University of Crete, Crete, Greece
For correspondence: tavernarakis@imbb.forth.gr
Bio-protocol author page: a3833
date: 1/20/2017, 916 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2108.

[Abstract] Eukaryotic cells contain various types of cytoplasmic, non-membrane bound ribonucleoprotein (RNP) granules that consist of non-translating mRNAs and a versatile set of associated proteins. One prominent type of RNP granules are Processing bodies (P bodies), which majorly harbors translationally inactive ...

Assay to Access Anthelmintic Activity of Small Molecule Drugs Using Caenohabidtis elegans as a Model

Authors: Viviane Sant’Anna
Viviane Sant’AnnaAffiliation: Laboratório de Biologia de Helmintos Otto Wucherer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Cidade Universitária, Bloco G, Ilha do Fundão, Rio de Janeiro, RJ, Brazil
Bio-protocol author page: a4018
Wanderley de Souza
Wanderley de SouzaAffiliation: Laboratório de Ultraestrutura Celular Herta Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Cidade Universitária, Bloco G, Ilha do Fundão, Rio de Janeiro, RJ, Brazil
Bio-protocol author page: a4019
 and Rossiane C. Vommaro
Rossiane C. VommaroAffiliation: Laboratório de Ultraestrutura Celular Herta Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Cidade Universitária, Bloco G, Ilha do Fundão, Rio de Janeiro, RJ, Brazil
For correspondence: vommaro@biof.ufrj.br
Bio-protocol author page: a4020
date: 1/20/2017, 836 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2113.

[Abstract] This protocol proposes to use the nematode Caenorhabditis elegans as a model to screen and study the anthelmintic activity of natural and synthetic compounds and to observe their effects on the morphology and the ultrastructure of the helminthes. Furthermore, C. elegans can be used to investigate the ...
1 2 3 4 5 

[Bio101] Common Worm Media and Buffers

Author: Fanglian He date: 4/5/2011, 23572 views, 10 Q&A
DOI: https://doi.org/10.21769/BioProtoc.55.

[Abstract] Here are recipes of some media and solutions often used in C. elegans research....

[Bio101] Lifespan Assay

Author: Fanglian He date: 4/5/2011, 14561 views, 3 Q&A
DOI: https://doi.org/10.21769/BioProtoc.57.

[Abstract] This assay is used to address aging-related questions in worms....

[Bio101] Total RNA Extraction from C. elegans

Author: Fanglian He date: 3/20/2011, 14454 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.47.

[Abstract] This protocol describes total RNA extraction from worms with or without using commercial RNA extraction kits....

[Bio101] Making Males of C. elegans

Author: Fanglian He date: 4/20/2011, 13664 views, 2 Q&A
DOI: https://doi.org/10.21769/BioProtoc.58.

[Abstract] Getting males from a hermaphrodite population. This is a modified version of protocol originally written by Michael Koelle at Yale University....

[Bio101] RNA Interference (RNAi) by Bacterial Feeding

Author: Fanglian He date: 4/20/2011, 13573 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.59.

[Abstract] There are 3 ways to perform RNAi in worms: microinjection, soaking and feeding. In the feeding protocol, RNAi is induced by cultivating worms on bacteria expressing gene-specific dsRNA. dsRNA is expressed in E. coli and ingested by worms. This protocol describes the feeding protocol to induce RNAi....

[Bio101] Synchronization of Worm

Author: Fanglian He date: 4/5/2011, 13113 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.56.

[Abstract] Attaining a large synchronized population of worms is desirable for use in some assays in order to eliminate variation in results due to age differences. Two ways to get synchronized worms include egg preparation via bleaching and egg lay. The former in general yields more progeny than the latter, however, ...

PCR-RFLP Genotyping of Point Mutations in Caenorhabditis elegans

Author: Peichuan Zhang
Peichuan ZhangAffiliation: Department of Biochemistry and Biophysics, University of California, San Francisco, USA
For correspondence: peichuan.zhang@ucsf.edu
Bio-protocol author page: a11
date: 3/20/2012, 12453 views, 3 Q&A
DOI: https://doi.org/10.21769/BioProtoc.128.

[Abstract] This protocol describes the basic principle of PCR/restriction digest genotyping of point mutations in worms, based on the principle of Restriction Fragment Length Polymorphism (RFLP) analysis. This type of genotyping is, particularly, useful when phenotypic analysis of animals carrying point mutations ...

[Bio101] Oil Red O Staining of Fixed Worm

Author: Fanglian He date: 7/5/2012, 12037 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.230.

[Abstract] Oil red O staining is used to assess major fat stores in C. elegans. This protocol is adapted from the Ashrafi Lab at the University of California-San Francisco....

[Bio101] DAPI Nuclear Staining of Live Worm

Author: Fanglian He date: 6/5/2011, 10442 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.77.

[Abstract] Adapted from the Villenueve Lab at Stanford University. This is a very simple method using ethanol fixation, but works very well....

[Bio101] Single Worm PCR

Author: Fanglian He date: 4/20/2011, 9873 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.60.

[Abstract] This protocol is used for genotyping single worms....
1 2 3 4 5