Welcome guest, Sign in

Home

Fungi

Fusarium graminearum Maize Stalk Infection Assay and Associated Microscopic Observation Protocol

Featured protocol,  Authors: Juan He
Juan HeAffiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
Bio-protocol author page: a3791
Tinglu Yuan
Tinglu Yuan Affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
Bio-protocol author page: a3792
 and Wei-Hua Tang
Wei-Hua TangAffiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
For correspondence: whtang@sibs.ac.cn
Bio-protocol author page: a2037
date: 12/5/2016, 32 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.2034.

Brief version appeared in PLOS Pathogens, Mar 2016
The ascomycete fungus Fusarium graminearum (previously also called Gibberella zeae) causes Gibberella stalk rot in maize (Zea mays) and results in lodging and serious yield reduction. To develop methods to assess the fungal growth and symptom development in maize stalks, we present here a protocol of maize stalk inoculation with conidiospores of fluorescent protein-tagged F. graminearumand microscopic observation of the stalk infection process. The inoculation protocol provides repeatable results in stalk rot symptom development, and allows tracking of fungal hyphal growth inside maize stalks at cellular scale.

Uptake Assay for Radiolabeled Peptides in Yeast

Featured protocol,  Authors: Melinda Hauser
Melinda HauserAffiliation: Department of Microbiology, University of Tennessee, Knoxville, USA
Bio-protocol author page: a3756
Houjian Cai
Houjian CaiAffiliation: Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, USA
Bio-protocol author page: a3755
Fred Naider
Fred NaiderAffiliation 1: Department of Chemistry and Macromolecular Assembly Institute, College of Staten Island of the City University of New York, Staten Island, New York, USA
Affiliation 2: Ph.D. Programs in Biochemistry and Chemistry, The Graduate Center of the City University of New York, New York, USA
Bio-protocol author page: a3757
 and Jeffrey M. Becker
Jeffrey M. BeckerAffiliation: Department of Microbiology, University of Tennessee, Knoxville, USA
For correspondence: jbecker@utk.edu
Bio-protocol author page: a3758
date: 11/20/2016, 117 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.2026.

Brief version appeared in Eukaryot Cell, Oct 2007
We describe an assay for measuring the uptake of radioactive peptides into the yeast Saccharomyces cerevisiae. The methods presented here can be adapted to measure a variety of substrates transported into any bacterial or fungal cell via specific carrier-mediated systems.

Halo Assay for Toxic Peptides and Other Compounds in Microorganisms

Featured protocol,  Authors: Houjian Cai
Houjian CaiAffiliation: Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, USA
Bio-protocol author page: a3755
Melinda Hauser
Melinda HauserAffiliation: Department of Microbiology, University of Tennessee, Knoxville, USA
Bio-protocol author page: a3756
Fred Naider
Fred NaiderAffiliation 1: Department of Chemistry and Macromolecular Assembly Institute, College of Staten Island of the City University of New York, Staten Island, USA
Affiliation 2: Ph.D. Programs in Biochemistry and Chemistry, The Graduate Center of the City University of New York, New York, USA
Bio-protocol author page: a3757
 and Jeffrey M. Becker
Jeffrey M. BeckerAffiliation: Department of Microbiology, University of Tennessee, Knoxville, USA
For correspondence: jbecker@utk.edu
Bio-protocol author page: a3758
date: 11/20/2016, 122 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.2025.

Brief version appeared in Eukaryot Cell, Oct 2007
We describe an assay for determination of toxicity in S. cerevisiae involving spotting of a toxic peptide on a lawn of yeast cells. This assay may be generalized to determine toxicity of a variety of compounds by substituting a putative toxic compound in place of the peptide. The general protocol may also be used to determine toxicity of any small compound toward another microorganism by replacing S. cerevisiae with the target microbe and modifying growth conditions accordingly.

Fusarium graminearum Maize Stalk Infection Assay and Associated Microscopic Observation Protocol

Authors: Juan He
Juan HeAffiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
Bio-protocol author page: a3791
Tinglu Yuan
Tinglu Yuan Affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
Bio-protocol author page: a3792
 and Wei-Hua Tang
Wei-Hua TangAffiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
For correspondence: whtang@sibs.ac.cn
Bio-protocol author page: a2037
date: 12/5/2016, 32 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.2034.

[Abstract] The ascomycete fungus Fusarium graminearum (previously also called Gibberella zeae) causes Gibberella stalk rot in maize (Zea mays) and results in lodging and serious yield reduction. To develop methods to assess the fungal growth and symptom development in maize stalks, we present here a protocol of ...

Uptake Assay for Radiolabeled Peptides in Yeast

Authors: Melinda Hauser
Melinda HauserAffiliation: Department of Microbiology, University of Tennessee, Knoxville, USA
Bio-protocol author page: a3756
Houjian Cai
Houjian CaiAffiliation: Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, USA
Bio-protocol author page: a3755
Fred Naider
Fred NaiderAffiliation 1: Department of Chemistry and Macromolecular Assembly Institute, College of Staten Island of the City University of New York, Staten Island, New York, USA
Affiliation 2: Ph.D. Programs in Biochemistry and Chemistry, The Graduate Center of the City University of New York, New York, USA
Bio-protocol author page: a3757
 and Jeffrey M. Becker
Jeffrey M. BeckerAffiliation: Department of Microbiology, University of Tennessee, Knoxville, USA
For correspondence: jbecker@utk.edu
Bio-protocol author page: a3758
date: 11/20/2016, 117 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.2026.

[Abstract] We describe an assay for measuring the uptake of radioactive peptides into the yeast Saccharomyces cerevisiae. The methods presented here can be adapted to measure a variety of substrates transported into any bacterial or fungal cell via specific carrier-mediated systems.

[Background] Di/tripeptides ...

Halo Assay for Toxic Peptides and Other Compounds in Microorganisms

Authors: Houjian Cai
Houjian CaiAffiliation: Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, USA
Bio-protocol author page: a3755
Melinda Hauser
Melinda HauserAffiliation: Department of Microbiology, University of Tennessee, Knoxville, USA
Bio-protocol author page: a3756
Fred Naider
Fred NaiderAffiliation 1: Department of Chemistry and Macromolecular Assembly Institute, College of Staten Island of the City University of New York, Staten Island, USA
Affiliation 2: Ph.D. Programs in Biochemistry and Chemistry, The Graduate Center of the City University of New York, New York, USA
Bio-protocol author page: a3757
 and Jeffrey M. Becker
Jeffrey M. BeckerAffiliation: Department of Microbiology, University of Tennessee, Knoxville, USA
For correspondence: jbecker@utk.edu
Bio-protocol author page: a3758
date: 11/20/2016, 122 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.2025.

[Abstract] We describe an assay for determination of toxicity in S. cerevisiae involving spotting of a toxic peptide on a lawn of yeast cells. This assay may be generalized to determine toxicity of a variety of compounds by substituting a putative toxic compound in place of the peptide. The general protocol may ...

An Assay to Study Botrytis cinerea-infected Grapevine Leaves Primed with Pseudomonas fluorescens

Authors: Charlotte Gruau
Charlotte GruauAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3539
Patricia Trotel-Aziz
Patricia Trotel-AzizAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3540
Bas Verhagen
Bas VerhagenAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3541
Sandra Villaume
Sandra VillaumeAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3542
Fanja Rabenoelina
Fanja RabenoelinaAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3543
Barbara Courteaux
Barbara CourteauxAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3544
Christophe Clément
Christophe ClémentAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3545
Fabienne Baillieul
Fabienne BaillieulAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3546
 and Aziz Aziz
Aziz AzizAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
For correspondence: aziz.aziz@univ-reims.fr
Bio-protocol author page: a3547
date: 10/5/2016, 318 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1943.

[Abstract] Grapevine (Vitis vinifera L.) is susceptible to an array of diseases among them the grey mold caused by the necrotrophic fungus Botrytis cinerea that decreases grape productivity and quality. To ensure a satisfactory yield and harvest quality numerous chemical fungicides are required, but they have ...

Flow Cytometry of Lung and Bronchoalveolar Lavage Fluid Cells from Mice Challenged with Fluorescent Aspergillus Reporter (FLARE) Conidia

Authors: Anupam Jhingran
Anupam JhingranAffiliation: Infectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, USA
Bio-protocol author page: a3498
Shinji Kasahara
Shinji KasaharaAffiliation: Infectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, USA
Bio-protocol author page: a3499
 and Tobias M Hohl
Tobias M HohlAffiliation 1: Infectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, USA
Affiliation 2: Immunology Program, Sloan Kettering Institute; Memorial Sloan Kettering Cancer Center, New York, USA
For correspondence: hohlt@mskcc.org
Bio-protocol author page: a3500
date: 9/20/2016, 358 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1927.

[Abstract] Aspergillus fumigatus is a ubiquitous fungal pathogen that forms airborne conidia. The process of restricting conidial germination into hyphae by lung leukocytes is critical in determining infectious outcomes. Tracking the outcome of conidia-host cell encounters in vivo is technically challenging and ...

59Fe Uptake Assays in Paracoccidioides Species

Authors: Daniel J. Kosman
Daniel J. KosmanAffiliation: Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, NY, USA
Bio-protocol author page: a3506
Elisa Flávia Luiz Cardoso Bailão
Elisa Flávia Luiz Cardoso BailãoAffiliation: Universidade Estadual de Goiás, Câmpus Henrique Santillo, Anápolis, Goiás, Brazil
Bio-protocol author page: a3507
Mirelle Garcia Silva-Bailão
Mirelle Garcia Silva-BailãoAffiliation 1: Universidade Federal de Goiás, Unidade Acadêmica Especial Ciências da Saúde, Jataí, Goiás, Brazil
Affiliation 2: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil
Bio-protocol author page: a3508
 and Célia Maria de Almeida Soares
Célia Maria de Almeida SoaresAffiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil
For correspondence: cmasoares@gmail.com
Bio-protocol author page: a3509
date: 9/20/2016, 309 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1930.

[Abstract] Iron is an essential micronutrient required for virtually all organisms. This fact is related to the ability of the transition metal to exist in two oxidation states, the reduced ferrous (Fe2+) and the oxidized ferric (Fe3+). Given the relative availability of aqueous iron (the element which constitutes ...

In vitro Cell Wall Stress Assay for Fusarium oxysporum

Authors: Elena Pérez-Nadales
Elena Pérez-NadalesAffiliation: Maimonides Biomedical Research Institute of Cordoba (IMIBIC), Reina Sofia University Hospital, University of Cordoba, Spain
For correspondence: elena.pereznadales@imibic.org
Bio-protocol author page: a3465
 and Antonio Di Pietro
Antonio Di PietroAffiliation: Departamento de Genética, Campus de Excelencia Agroalimentario ceiA3, Universidad de Cordoba, Spain
Bio-protocol author page: a3466
date: 9/5/2016, 383 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1915.

[Abstract] In this protocol we describe a cell wall stress assay for the fungal pathogen F. oxysporum, based on exposure to the two anionic dyes Calcofluor White (CFW) and Congo Red (CR). Both compounds have been used to exert stress upon the fungal cell wall in vitro (Perez-Nadales and Di Pietro, 2015; Perez-Nadales ...

A Highly Efficient Method for Measuring Oxygen Consumption Rate in Fusarium graminearum

Authors: Daniel Gebhard
Daniel GebhardAffiliation: Department of Life Sciences, Albstadt-Sigmaringen University of Applied Sciences, Sigmaringen, Germany
Bio-protocol author page: a3383
Jakob Bönnighausen
Jakob BönnighausenAffiliation: Biocenter Klein Flottbek, Department of Molecular Phytopathology, University of Hamburg, Hamburg, Germany
Bio-protocol author page: a3384
Jörg Bergemann
Jörg BergemannAffiliation: Department of Life Sciences, Albstadt-Sigmaringen University of Applied Sciences, Sigmaringen, Germany
Bio-protocol author page: a3385
Wilhelm Schäfer
Wilhelm SchäferAffiliation: Biocenter Klein Flottbek, Department of Molecular Phytopathology, University of Hamburg, Hamburg, Germany
Bio-protocol author page: a3386
 and Jörg Bormann
Jörg BormannAffiliation: Biocenter Klein Flottbek, Department of Molecular Phytopathology, University of Hamburg, Hamburg, Germany
For correspondence: joerg.bormann@uni-hamburg.de
Bio-protocol author page: a3387
date: 8/5/2016, 474 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1887.

[Abstract] The filamentous ascomycete Fusarium graminearum is the causal agent of Fusarium head blight, a devastating disease of cereals with a worldwide distribution. Fusarium graminearum infections result in a quantitative yield reduction by impairing the growth of the kernels, and a qualitative reduction by ...

Antifungal and Zearalenone Inhibitory Activity of Ocimum sanctum L. Essential Oil on Fusarium graminearum Determined by UHPLC and RT-qPCR

Authors: Naveen Kumar Kalagatur
Naveen Kumar KalagaturAffiliation: Food Microbiology Division, Defence Food Research Laboratory, Mysore, Karnataka, India
Bio-protocol author page: a3404
Nirmaladevi Dhamodaran
Nirmaladevi DhamodaranAffiliation: Department of Microbiology and Biotechnology, Bangalore University, Bangalore, Karnataka, India
Bio-protocol author page: a3405
Chandranayaka Siddaiah
Chandranayaka SiddaiahAffiliation: Department of Biotechnology, Mysore University, Mysore, Karnataka, India
Bio-protocol author page: a3406
Venkataramana Mudili
Venkataramana MudiliAffiliation: Toxicology and Immunology Division, DRDO-BU-Centre for Life Sciences, Coimbatore, Tamil Nadu, India
For correspondence: ramana.micro@gmail.com
Bio-protocol author page: a3407
 and Murali Harishchandra Sreepathi
Murali Harishchandra SreepathiAffiliation: Food Microbiology Division, Defence Food Research Laboratory, Mysore, Karnataka, India
Bio-protocol author page: a3448
date: 8/5/2016, 455 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1893.

[Abstract] Fusarium graminearum has been given special attention in the context of agricultural commodities due to its ability to grow in diverse climatic conditions, and to produce different mycotoxins including zearalenone (ZEA) and type-B trichothecenes, which cause ill health effects on humans, animals and ...

Quantification of Chitinase Activity in Fusarium oxysporum

Authors: Carmen Ruiz-Roldan
Carmen Ruiz-RoldanAffiliation: Departamento de Genetica, Universidad de Cordoba, Spain; Campus de Excelencia Agroalimentario, Cordoba, Spain
For correspondence: ge2rurom@uco.es
Bio-protocol author page: a3318
 and M. Isabel G. Roncero
M. Isabel G. RonceroAffiliation: Departamento de Genetica, Universidad de Cordoba, Spain; Campus de Excelencia Agroalimentario, Cordoba, Spain
Bio-protocol author page: a3319
date: 8/5/2016, 512 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1882.

[Abstract] Fungal morphogenetic development requires modification and plasticity of the cell wall, which implies synthesis and remodelling of its components, including chitin and glucan. Thus chitinase and glucanase activities are crucial for cell-wall biogenesis and cell division. Quantification of chitinase ...
1 2 3 4 5 6 7 8 9 10 

In vitro Protein Kinase Assay

Author: Yuehua Wei
Yuehua WeiAffiliation: Department of Pharmacology, Cancer Institute of New Jersey, UMDNJ Robert Wood Johnson Medical School, Piscataway, USA
For correspondence: weiyh.sjtu.edu@gmail.com
Bio-protocol author page: a49
date: 6/5/2012, 20290 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.212.

[Abstract] This protocol will describe experimental procedures for an in vitro kinase assay of the yeast protein kinase Sch9. This protocol can be tailored to detect kinase activity of other yeast protein kinase....

[Bio101] Making Yeast Competent Cells and Yeast Cell Transformation

Author: Yongxian Lu
Yongxian LuAffiliation: Carnegie Institution for Science, Stanford University, Stanford, USA
For correspondence: yxlu@stanford.edu
Bio-protocol author page: a28
date: 7/20/2011, 19512 views, 2 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.96.

[Abstract] This is a quite simple but reliable protocol to make very high transformation efficiency yeast competent cells. By express your gene of interest, protein function can be studied in yeast cells....

[Bio101] Protocol for Whole Cell Lysis of Yeast

Author: Zongtian Tong
Zongtian TongAffiliation: Department of Cell Biology, Center for Metabolism and Obesity Research, Johns Hopkins School of Medicine, Baltimore, USA
For correspondence: tongzong@gmail.com
Bio-protocol author page: a14
date: 1/5/2011, 17156 views, 4 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.14.

[Abstract] This protocol describes how to perform lysis on whole yeast cell samples using NaOH. The lysed cells can then be used for downstream applications such as the extraction of total proteins. ...

Spot Assay for Yeast

Author: Zongtian Tong
Zongtian TongAffiliation: Department of Cell Biology, Center for Metabolism and Obesity Research, Johns Hopkins School of Medicine, Baltimore, USA
For correspondence: tongzong@gmail.com
Bio-protocol author page: a14
date: 1/5/2012, 16693 views, 3 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.16.

[Abstract] This protocol can be used to compare the cell growth rate of yeast under different growth conditions. It involves the serial dilution and spotting of yeast colonies....

[Bio101] Yeast Vacuole Staining with FM4-64

Author: Zongtian Tong
Zongtian TongAffiliation: Department of Cell Biology, Center for Metabolism and Obesity Research, Johns Hopkins School of Medicine, Baltimore, USA
For correspondence: tongzong@gmail.com
Bio-protocol author page: a14
date: 1/5/2011, 13532 views, 1 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.18.

[Abstract] The lipophilic probe, FM 4-64 does not fluoresce much in water but fluoresces strongly after binding to the outer plasma membrane, providing clear and distinguishable plasma membrane staining. The binding is rapid and reversible. In this protocol vacuoles in yeast cells are stained with the FM4-64 dye, ...

[Bio101] How to Use an Avestin Emulsiflex C3 Homogenizer to Disrupt Cells

Author: Zongtian Tong
Zongtian TongAffiliation: Department of Cell Biology, Center for Metabolism and Obesity Research, Johns Hopkins School of Medicine, Baltimore, USA
For correspondence: tongzong@gmail.com
Bio-protocol author page: a14
date: 1/5/2011, 10717 views, 1 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.11.

[Abstract] The EmulsiFlex-C3 homogenizer is powered by an electric motor. The pump does not require a compressor for it to run. This equipment can be used to disrupt cells at a large scale. The EmulsiFlex-C3 has a fixed flow-through capacity of 3 L/h. It has the ability to process samples as small as 10 ml. The ...

[Bio101] Large Scale Native Affinity Purifications of Solubilized Membrane Proteins from Yeast

Author: Zongtian Tong
Zongtian TongAffiliation: Department of Cell Biology, Center for Metabolism and Obesity Research, Johns Hopkins School of Medicin, Baltimore , USA
For correspondence: tongzong@gmail.com
Bio-protocol author page: a14
date: 1/5/2011, 10425 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.12.

[Abstract] This protocol can be used to purify membrane proteins from yeast samples under native conditions at a large scale. This protocol has been developed primarily for FLAG-tagged proteins. This protocol can however be slightly modified and applied to other tags, such as GST or HA....

[Bio101] Small Scale Native Affinity Purifications of Solubilized Membrane Proteins from Yeast

Author: Zongtian Tong
Zongtian TongAffiliation: Department of Cell Biology, Center for Metabolism and Obesity Research, Johns Hopkins School of Medicine, Baltimore, USA
For correspondence: tongzong@gmail.com
Bio-protocol author page: a14
date: 1/5/2011, 9866 views, 1 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.15.

[Abstract] In this protocol, we show how to purify membrane proteins from yeast using affinity purification under native conditions at a small scale. ...

Illumina Sequencing Library Construction from ChIP DNA

Author: Wei Zheng
Wei ZhengAffiliation: Keck Biotech Services, Yale University, New Haven, USA
For correspondence: wei.zheng.madison@gmail.com
Bio-protocol author page: a10
date: 2/20/2012, 9219 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.91.

[Abstract] The Illumina sequencing platform is very popular among next-generation sequencing platforms. However, the DNA sequencing library construction kit provided by Illumina is considerably expensive. The protocol described here can be used to construct high-quality sequencing libraries from chromatin immunoprecipitated ...

Co-immunoprecipitation in Yeast

Author: Olesya O. Panasenko
Olesya O. PanasenkoAffiliation: Department of Microbiology and Molecular Medicine, University of Geneva, Faculty of Medicine, CMU, Geneva, Switzerland
For correspondence: olesya.panasenko@unige.ch
Bio-protocol author page: a88
date: 8/20/2012, 8687 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.250.

[Abstract] This protocol describes investigation of protein-protein interactions in baker yeast by co-immunoprecipitation (CoIP). CoIP is a technique to identify physiologically relevant protein-protein interactions in the cell. The interesting protein can be isolated out of solution using antibody that specifically ...
1 2 3 4 5 6 7 8 9 10