Welcome guest, Sign in

Home

Other Vertebrates

Identification of Methylated Deoxyadenosines in Genomic DNA by dA6m DNA Immunoprecipitation

Authors: Magdalena J. Koziol
Magdalena J. KoziolAffiliation 1: Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK
Affiliation 2: Department of Zoology, University of Cambridge, Cambridge, UK
For correspondence: mjk39@cam.ac.uk
Bio-protocol author page: a3666
Charles R. Bradshaw
Charles R. BradshawAffiliation: Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK
Bio-protocol author page: a3667
George E. Allen
George E. AllenAffiliation: Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK
Bio-protocol author page: a3668
Ana S. H. Costa
Ana S. H. CostaAffiliation: Medical Research Council Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Cambridge, UK
Bio-protocol author page: a3669
 and Christian Frezza
Christian FrezzaAffiliation: Medical Research Council Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Cambridge, UK
Bio-protocol author page: a3670
date: 11/5/2016, 179 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1990.

[Abstract] dA6m DNA immunoprecipitation followed by deep sequencing (DIP-Seq) is a key tool in identifying and studying the genome-wide distribution of N6-methyldeoxyadenosine (dA6m). The precise function of this novel DNA modification remains to be fully elucidated, but it is known to be absent from transcriptional ...

The Chick Embryo Chorioallantoic Membrane as an in vivo Model to Study Metastasis

Authors: Piero Crespo
Piero CrespoAffiliation: Instituto de Biomedicina y Biotecnología de Cantabria (IBBTEC), Consejo Superior de Investigaciones Científicas (CSIC)-Universidad de Cantabria (UC), Santander, Cantabria, Spain
Bio-protocol author page: a3590
 and Berta Casar
Berta CasarAffiliation: Instituto de Biomedicina y Biotecnología de Cantabria (IBBTEC), Consejo Superior de Investigaciones Científicas (CSIC)-Universidad de Cantabria (UC), Santander, Cantabria, Spain
For correspondence: casarb@unican.es
Bio-protocol author page: a3591
date: 10/20/2016, 265 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1962.

[Abstract] Metastasis is a complex process that includes several steps: neoplastic progression, angiogenesis, cell migration and invasion, intravasation into nearby blood vessels, survival in the circulatory system, extravasation followed by homing into distant tissues, the formation of micrometastases, and finally ...

Chick Neural Tube Explant Culture

Authors: Zhanna Alekseenko
Zhanna AlekseenkoAffiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
Bio-protocol author page: a2597
Elisabet Andersson
Elisabet AnderssonAffiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
Bio-protocol author page: a2598
 and José M. Dias
José M. DiasAffiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
For correspondence: jose.dias@ki.se
Bio-protocol author page: a2599
date: 10/5/2015, 1194 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1608.

[Abstract] The neural tube explant culture technique allows in vitro culturing of small pieces of neural tissue isolated from e.g. chick or mouse embryonic tissue in a matrix of collagen for defined periods of time. This method can be used to study the effects of defined molecules on developmental processes such ...

Immunolocalization of Proteins in Corals: the V-type H+-ATPase Proton Pump

Authors: Katie L. Barott
Katie L. BarottAffiliation: Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, USA
Bio-protocol author page: a2501
 and Martin Tresguerres
Martin TresguerresAffiliation: Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, USA
For correspondence: mtresguerres@ucsd.edu
Bio-protocol author page: a2502
date: 9/5/2015, 1590 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1573.

[Abstract] Here we describe the immunolocalization of a membrane-bound proton pump, the V-type H+-ATPase (VHA), in tissues and isolated cells of scleractinian corals. Immunolocalization of coral proteins requires additional steps not required for various model organisms, such as decalcification of the coral skeleton ...

Electroporation of Embryonic Chick Eyes

Authors: Agustín Luz-Madrigal
Agustín Luz-MadrigalAffiliation 1: Department of Biology, Miami University and Center for Visual Sciences at Miami University (CVSMU), Oxford, USA
Affiliation 2: Department of Biology, University of Dayton and Center for Tissue Regeneration and Engineering at the University of Dayton (TREND), Dayton, USA
Bio-protocol author page: a2286
Erika Grajales-Esquivel
Erika Grajales-EsquivelAffiliation: Department of Biology, Miami University and Center for Visual Sciences at Miami University (CVSMU), Oxford, USA
Bio-protocol author page: a2287
 and Katia Del Rio-Tsonis
Katia Del Rio-TsonisAffiliation: Department of Biology, Miami University and Center for Visual Sciences at Miami University (CVSMU), Oxford, USA
For correspondence: delriok@miamioh.edu
Bio-protocol author page: a2288
date: 6/20/2015, 1894 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1498.

[Abstract] The chick embryo has prevailed as one of the major models to study developmental biology, cell biology and regeneration. From all the anatomical features of the chick embryo, the eye is one of the most studied. In the chick embryo, the eye develops between 26 and 33 h after incubation (Stages 8-9, Hamburger ...

Open-book Preparations from Chick Embryos and DiI Labeling of Commissural Axons

Authors: Nicole H. Wilson
Nicole H. WilsonAffiliation: Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland
For correspondence: nicole.wilson@imls.uzh.ch
Bio-protocol author page: a1464
 and Esther T. Stoeckli
Esther T. StoeckliAffiliation: Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland
For correspondence: esther.stoeckli@imls.uzh.ch
Bio-protocol author page: a1465
date: 7/5/2014, 2852 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1176.

[Abstract] Successful neural circuit formation relies on the accurate navigation of axons towards their targets during development. Axons are guided by a combination of short-range and long-range, attractive and repulsive cues. The commissural axons of the developing spinal cord have provided an informative in ...

Acquisition of Leftward Flow in Xenopus laevis

Authors: Thomas Thumberger
Thomas ThumbergerAffiliation: Centre for Organismal Studies (COS), Heidelberg University, Heidelberg, Germany
For correspondence: thomas.thumberger@cos.uni-heidelberg.de
Bio-protocol author page: a1037
 and Martin Blum
Martin BlumAffiliation: Institute of Zoology, University of Hohenheim, Stuttgart, Germany
For correspondence: martin.blum@uni-hohenheim.de
Bio-protocol author page: a457
date: 12/5/2013, 2271 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.996.

[Abstract] In Xenopus, the left-right axis is established following an extracellular vectorial leftward flow driven by monocilia at the gastrocoel roof plate (GRP) during late gastrulation / neurulation (Schweickert et al., 2007). As the GRP lies inside the developing archenteron, imaging of flow is challenging. ...

Implantation of Dkk-1-soaked Beads into the Neural Tube of Chicken Embryos

Authors: Almudena Martinez-Ferre
Almudena Martinez-FerreAffiliation: Institute of Neurosciences, Miguel Hernández University-Spanish National Research Council, San Juan, Alicante, Spain
Bio-protocol author page: a967
Maria Navarro-Garberí
Maria Navarro-GarberíAffiliation: Institute of Neurosciences, Miguel Hernández University-Spanish National Research Council, San Juan, Alicante, Spain
Bio-protocol author page: a968
Carlos Bueno
Carlos BuenoAffiliation: Institute of Neurosciences, Miguel Hernández University-Spanish National Research Council, San Juan, Alicante, Spain
Bio-protocol author page: a969
 and Salvador Martinez
Salvador MartinezAffiliation: Institute of Neurosciences, Miguel Hernández University-Spanish National Research Council, San Juan, Alicante, Spain
For correspondence: smartinez@umh.es
Bio-protocol author page: a970
date: 11/5/2013, 3540 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.963.

[Abstract] Chick embryos are known to be a powerful system to test gene function due to the “in vivo” accessibility, short time for results retrieval and the possibility to perform a large number of experiments. Synthetic micro-beads soaked in morphogenetic signals or receptor inhibitors can be implanted in selective ...

In vivo Chick Chorioallantoic Membrane (CAM) Angiogenesis Assays

Authors: Zhenguo Chen
Zhenguo ChenAffiliation: Department of Cell Biology, Southern Medical University, Guangzhou, China
Bio-protocol author page: a850
Zhihua Wen
Zhihua WenAffiliation: Department of Cell Biology, Southern Medical University, Guangzhou, China
Bio-protocol author page: a851
 and Xiaochun Bai
Xiaochun BaiAffiliation: Department of Cell Biology, Southern Medical University, Guangzhou, China
For correspondence: baixc15@smu.edu.cn
Bio-protocol author page: a852
date: 9/20/2013, 9629 views, 3 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.913.

[Abstract] Angiogenesis is the process of formation of new blood vessels from pre-existing vessels or endothelial cell progenitors. It plays a crucial role in tumor growth and metastasis. Tumor angiogenesis have been widely studied as an important target for suppressing tumor growth and metastasis. Here, we describe ...
1 

In vivo Chick Chorioallantoic Membrane (CAM) Angiogenesis Assays

Authors: Zhenguo Chen
Zhenguo ChenAffiliation: Department of Cell Biology, Southern Medical University, Guangzhou, China
Bio-protocol author page: a850
Zhihua Wen
Zhihua WenAffiliation: Department of Cell Biology, Southern Medical University, Guangzhou, China
Bio-protocol author page: a851
 and Xiaochun Bai
Xiaochun BaiAffiliation: Department of Cell Biology, Southern Medical University, Guangzhou, China
For correspondence: baixc15@smu.edu.cn
Bio-protocol author page: a852
date: 9/20/2013, 9629 views, 3 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.913.

[Abstract] Angiogenesis is the process of formation of new blood vessels from pre-existing vessels or endothelial cell progenitors. It plays a crucial role in tumor growth and metastasis. Tumor angiogenesis have been widely studied as an important target for suppressing tumor growth and metastasis. Here, we describe ...

Implantation of Dkk-1-soaked Beads into the Neural Tube of Chicken Embryos

Authors: Almudena Martinez-Ferre
Almudena Martinez-FerreAffiliation: Institute of Neurosciences, Miguel Hernández University-Spanish National Research Council, San Juan, Alicante, Spain
Bio-protocol author page: a967
Maria Navarro-Garberí
Maria Navarro-GarberíAffiliation: Institute of Neurosciences, Miguel Hernández University-Spanish National Research Council, San Juan, Alicante, Spain
Bio-protocol author page: a968
Carlos Bueno
Carlos BuenoAffiliation: Institute of Neurosciences, Miguel Hernández University-Spanish National Research Council, San Juan, Alicante, Spain
Bio-protocol author page: a969
 and Salvador Martinez
Salvador MartinezAffiliation: Institute of Neurosciences, Miguel Hernández University-Spanish National Research Council, San Juan, Alicante, Spain
For correspondence: smartinez@umh.es
Bio-protocol author page: a970
date: 11/5/2013, 3540 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.963.

[Abstract] Chick embryos are known to be a powerful system to test gene function due to the “in vivo” accessibility, short time for results retrieval and the possibility to perform a large number of experiments. Synthetic micro-beads soaked in morphogenetic signals or receptor inhibitors can be implanted in selective ...

Open-book Preparations from Chick Embryos and DiI Labeling of Commissural Axons

Authors: Nicole H. Wilson
Nicole H. WilsonAffiliation: Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland
For correspondence: nicole.wilson@imls.uzh.ch
Bio-protocol author page: a1464
 and Esther T. Stoeckli
Esther T. StoeckliAffiliation: Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland
For correspondence: esther.stoeckli@imls.uzh.ch
Bio-protocol author page: a1465
date: 7/5/2014, 2852 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1176.

[Abstract] Successful neural circuit formation relies on the accurate navigation of axons towards their targets during development. Axons are guided by a combination of short-range and long-range, attractive and repulsive cues. The commissural axons of the developing spinal cord have provided an informative in ...

Acquisition of Leftward Flow in Xenopus laevis

Authors: Thomas Thumberger
Thomas ThumbergerAffiliation: Centre for Organismal Studies (COS), Heidelberg University, Heidelberg, Germany
For correspondence: thomas.thumberger@cos.uni-heidelberg.de
Bio-protocol author page: a1037
 and Martin Blum
Martin BlumAffiliation: Institute of Zoology, University of Hohenheim, Stuttgart, Germany
For correspondence: martin.blum@uni-hohenheim.de
Bio-protocol author page: a457
date: 12/5/2013, 2271 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.996.

[Abstract] In Xenopus, the left-right axis is established following an extracellular vectorial leftward flow driven by monocilia at the gastrocoel roof plate (GRP) during late gastrulation / neurulation (Schweickert et al., 2007). As the GRP lies inside the developing archenteron, imaging of flow is challenging. ...

Electroporation of Embryonic Chick Eyes

Authors: Agustín Luz-Madrigal
Agustín Luz-MadrigalAffiliation 1: Department of Biology, Miami University and Center for Visual Sciences at Miami University (CVSMU), Oxford, USA
Affiliation 2: Department of Biology, University of Dayton and Center for Tissue Regeneration and Engineering at the University of Dayton (TREND), Dayton, USA
Bio-protocol author page: a2286
Erika Grajales-Esquivel
Erika Grajales-EsquivelAffiliation: Department of Biology, Miami University and Center for Visual Sciences at Miami University (CVSMU), Oxford, USA
Bio-protocol author page: a2287
 and Katia Del Rio-Tsonis
Katia Del Rio-TsonisAffiliation: Department of Biology, Miami University and Center for Visual Sciences at Miami University (CVSMU), Oxford, USA
For correspondence: delriok@miamioh.edu
Bio-protocol author page: a2288
date: 6/20/2015, 1894 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1498.

[Abstract] The chick embryo has prevailed as one of the major models to study developmental biology, cell biology and regeneration. From all the anatomical features of the chick embryo, the eye is one of the most studied. In the chick embryo, the eye develops between 26 and 33 h after incubation (Stages 8-9, Hamburger ...

Immunolocalization of Proteins in Corals: the V-type H+-ATPase Proton Pump

Authors: Katie L. Barott
Katie L. BarottAffiliation: Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, USA
Bio-protocol author page: a2501
 and Martin Tresguerres
Martin TresguerresAffiliation: Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, USA
For correspondence: mtresguerres@ucsd.edu
Bio-protocol author page: a2502
date: 9/5/2015, 1590 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1573.

[Abstract] Here we describe the immunolocalization of a membrane-bound proton pump, the V-type H+-ATPase (VHA), in tissues and isolated cells of scleractinian corals. Immunolocalization of coral proteins requires additional steps not required for various model organisms, such as decalcification of the coral skeleton ...

Chick Neural Tube Explant Culture

Authors: Zhanna Alekseenko
Zhanna AlekseenkoAffiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
Bio-protocol author page: a2597
Elisabet Andersson
Elisabet AnderssonAffiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
Bio-protocol author page: a2598
 and José M. Dias
José M. DiasAffiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
For correspondence: jose.dias@ki.se
Bio-protocol author page: a2599
date: 10/5/2015, 1194 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1608.

[Abstract] The neural tube explant culture technique allows in vitro culturing of small pieces of neural tissue isolated from e.g. chick or mouse embryonic tissue in a matrix of collagen for defined periods of time. This method can be used to study the effects of defined molecules on developmental processes such ...

The Chick Embryo Chorioallantoic Membrane as an in vivo Model to Study Metastasis

Authors: Piero Crespo
Piero CrespoAffiliation: Instituto de Biomedicina y Biotecnología de Cantabria (IBBTEC), Consejo Superior de Investigaciones Científicas (CSIC)-Universidad de Cantabria (UC), Santander, Cantabria, Spain
Bio-protocol author page: a3590
 and Berta Casar
Berta CasarAffiliation: Instituto de Biomedicina y Biotecnología de Cantabria (IBBTEC), Consejo Superior de Investigaciones Científicas (CSIC)-Universidad de Cantabria (UC), Santander, Cantabria, Spain
For correspondence: casarb@unican.es
Bio-protocol author page: a3591
date: 10/20/2016, 265 views, 0 Q&A, How to cite
DOI: https://doi.org/10.21769/BioProtoc.1962.

[Abstract] Metastasis is a complex process that includes several steps: neoplastic progression, angiogenesis, cell migration and invasion, intravasation into nearby blood vessels, survival in the circulatory system, extravasation followed by homing into distant tissues, the formation of micrometastases, and finally ...
1