The miRNeasy Mini Kit was used for miRNA isolation, according to the manufacturer’s instructions (Qiagen, Hilden, Germany). The microarray analysis was performed at the Microarray-Analytic Center (Medical Faculty, Mannheim, Germany), using the Affymetrix GeneChip miRNA 4.0 Array with a total of 30,424 microRNAs (Thermo Fisher Scientific, Dreieich, Germany).
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