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Virus titers of virus stocks and homogenized tissue samples were determined by end-point titration in eggs and/or MDCK cells. For end-point viral titration in eggs, 10-fold serial dilutions of each sample were inoculated into eggs. Sixty hours after inoculation, fluid from the allantoic cavity was collected and tested for the ability to agglutinate chicken erythrocytes as an indicator of viral replication. Infectious virus titers are reported as log10 EID50/ml, and were calculated from three replicates by the method of Reed-Muench.
Copyright and License information: ©2019 Xu, Li, Yang, Wang, Jia, Han, Wang and Zhu.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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