Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
2.12. Subcutaneous xenograft model
Ask a
question
Favorite
Female nude mice (5–6 weeks) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). A total of 5 × 106 HCT116 and Hep3B cells were subcutaneously inoculated to nude mice. When the tumors reached a mean group size of about 100 mm3, mice were randomly divided into four groups receiving sodium carboxymethyl cellulose (CMCNa) as vehicle, ARS (50 mg/kg), SAHA (50 mg/kg), and combination (50 mg/kg ARS plus 50 mg/kg SAHA) daily by intragastric administration for 3 weeks. Tumor volume was calculated as (length × width2)/2.
Copyright and License information: ©2019 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
