Preparation of PEI/siRNA complex

JP Jingtong Pan
WR Wenyi Ruan
MQ Mengyao Qin
YL Yueming Long
TW Tao Wan
KY Kaiyue Yu
YZ Yuanhao Zhai
CW Chuanbin Wu
YX Yuehong Xu
request Request a Protocol
ask Ask a question
Favorite

siRNA was added to the same volume of PEI solution at the N/P ratio of 10:1, and the mixture was incubated at room temperature for 30 min to obtain PEI/siRNA complex. The diameter and potential of the complex determined with Dynamic Light Scattering (Malvern Instruments NanoZS90, Worcestershire, UK) were 135.1 ± 5.2 nm and 34.6 ± 2.2 mV, respectively.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A