siRNA was added to the same volume of PEI solution at the N/P ratio of 10:1, and the mixture was incubated at room temperature for 30 min to obtain PEI/siRNA complex. The diameter and potential of the complex determined with Dynamic Light Scattering (Malvern Instruments NanoZS90, Worcestershire, UK) were 135.1 ± 5.2 nm and 34.6 ± 2.2 mV, respectively.
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