3.13. Tumor xenograft model

Female (NOD) SCID mice (Jackson Laboratory) aged 6-8 weeks were used to establish tumor xenograft models. Experiments were conducted according to institutional animal care and use committee (IACUC) policy. ARK1shSCRM cells (1×10⁷) were resuspended in 1 mg/mL Matrigel (Corning, Corning, NY) and injected subcutaneously onto the flank. After tumor engraftment, tumors were measured every three days with calipers and tumor volume was calculated using the equation V=l*w*π/6 (l=longest diameter, w=shortest, perpendicular diameter). Relative tumor volume for control and treatment conditions (V_C or V_T was calculated by normalizing tumor volume at day 14 over tumor volume at day 1. Relative tumor growth (V_T / V_C) was then calculated for each treatment condition.