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RNA extraction, cDNA synthesis and real time PCR (RT-PCR)
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RNA extraction, cDNA synthesis and real time PCR were performed as previously described [26]. RT-PCR primers were shown in Supplementary Table 1. Porcine TATA-binding protein 1 (TBP1) gene was used as an internal control [49] to normalize the RT-PCR efficiency and to quantify the expression of the genes in wild-type and Mstn −/− pigs.
Copyright and License information: ©2017 Cai et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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