Oil Red O staining of liver tissue

Oil Red O staining was performed following a standard protocol. Frozen liver sections (6-µm thick) were cut and dried. Samples were washed with 50% ethanol, stained with Oil Red O for 8 min, differentiated with 50% ethanol, rinsed with tap water, stained with hematoxylin, rinsed with tap water and mounted with glycerin jelly. Sections were subsequently examined and images were captured using a light microscope linked to a digital CCD camera at magnification, ×200.