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Measurement of oxidative burst
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The oxidative burst in leukocytes was measured in heparinized whole blood from WT and nfkb1−/− mice using Phagoburst kit (Glicotope Biotechnology) according to manufacturer’s instructions. Using flow cytometry this kit allows the detection of percentage of phagocytic cells which produce reactive oxidants (conversion of DHR123 to R123) and their enzymatic activity (amount of R123 per cell) against several stimuli opsonized bacteria (E. coli), Phorbol 12-myristate 13-acetate (PMA) and N-Formyl-Met-Leu-Phe (fMLP).
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Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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