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RNA isolation and mRNA-seq library construction
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Inflorescence collection and total RNA isolation were as described above. Total RNA from each genotype was used to generate mRNA-seq libraries using the NEBNext Ultra RNA Library Prep Kit (New England Biolabs, E7530). All size selection and clean-up steps were performed using AMPure XP (Beckman Coulter, A63881). The resulting libraries were pooled and sequenced (paired-end 150 bp) on a HiSeq 2500 instrument (Illumina).
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