The synthesized formulations' hydrodynamic diameter and size distribution, MSNs-TQ and LB-MSNs, were determined using the Zetasizer (Nano ZS, Malvern Instruments, UK). The sample was analyzed in quartz cuvettes, the temperature was set to 25 °C, and the viscosity and refractive index values used were that of pure water. This instrument utilizes laser diffraction to detect scattered laser light at a 90° angle and ambient temperature for diluted samples. A Polydispersity Index (PDI) is also provided as part of this approach, which provides information on the sample's polydispersity.
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