ELISpot assay, intracellular cytokine staining (ICS) and flow cytometry

JM Ji Ma
MY Michael Bright Yakass
SJ Sander Jansen
BM Bert Malengier-Devlies
DL Dominique Van Looveren
LS Lorena Sanchez-Felipe
TV Thomas Vercruysse
BW Birgit Weynand
MJ Mahadesh Prasad Arkalagud Javarappa
OQ Osbourne Quaye
PM Patrick Matthys
TR Tania Roskams
JN Johan Neyts
HT Hendrik Jan Thibaut
KD Kai Dallmeier
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Assessment of YFV- and SARS-CoV-2-specific cell-mediated immunity (CMI) by ELISpot and Flow cytometry with ICS was performed as established and described before.18,20 All peptides and reagents were prepared accordingly. The gating strategy used is outlined in Figure S1f.

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