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Generation and Culture of Bone Marrow-Derived Macrophages (BMDMs)
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1640-RPMI (Gibco, Grand Island, NY) containing 10% FBS and 1% penicillin-streptomycin solution (Gibco, Grand Island, NY) was prepared as complete medium. The bone marrow cells were extracted from the lower limbs of female C57BL/6 mice aged 6–8 weeks. After the removal of RBCs, bone marrow cells were seeded and cultured in 100 mm Petri dishes in complete medium added with 50 ng/mL macrophage colony-stimulating factor (Peprotech, Rocky Hill, New Jersey) at 37°C for a continuous 7 days to obtain mature BMDMs.
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