4.8. In Vitro Drug Release Studies Using a Dialysis Method in PBS (pH 5.4 and pH 7.4)

In vitro drug release studies were performed using the dialysis method [42]. An aliquot (5 mL) of each individual sample was placed in cellulose membrane dialysis tubing (MWCO 14,000). The mixtures were subjected to rotary machine stirring at 100 RPM in 50 mL of 40% methanol PBS pH (5.4) and pH (7.4) media at 37 ± 0.5 °C. At various time intervals, an aliquot of (3 mL) was withdrawn from the released media and was replaced with an equivalent volume of fresh media. Triplicate samples (n = 3) of each PAMAM generation dendrimer were measured. Meanwhile, the in vitro dissolution study for the reference tablet was examined using Pharma test (DT 70, Germany) dissolution apparatus, utilizing type II apparatus (paddle) at stirring speed of 75 RPM in 1000 mL of 0.01 N HCl, and compared with 0.02% Tween 80 in 0.01 N HCl [44]. The amount of ERL released from each medium was then analyzed with HPLC-UV [24].