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Vectors bearing DCN-shRNA and control shRNA were purchased from (Santa Cruz). The transfections were performed utilizing the Lipofectamine 3000 Transfection Kit (Invitrogen) following the recommended protocol. Briefly, exponentially growing cells were transfected for 3 days. Puromycin (2 µg/mL) was utilized to select the transfected cells.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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