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The CHX chase assay enables to observe degradation kinetics of proteins and therefore is a good tool to study protein stability [50]. HCT116 cells were transfected with EV/USP36 OE or treated with vehicle/CBF. Afterward, cells were cultivated in the medium containing 20 μg/mL of CHX. At 0, 4, 8, and 12 h, cells were collected for detecting the protein levels with western blot.
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