Rescue Experiment and Double Knockdown Experiment

The method of this experiment was referred to the previous research (28). PRDM1 rescue experiment was conducted in NLRP12-FLAG-expressing dTHP1 cells which were transfected with siRNAs. In brief, dTHP1 cells were transiently transfected siRNAs with by Lipo2000 (Invitrogen, CA, USA) for 24h, then PRDM1-Flag-expressing vectors were transfected into the cells using Lipo2000 (Invitrogen, CA, USA) for 24h. After 72h post-infection, the cells were harvested for western blot analysis. NLRP12 rescue experiment were similar to those described in PRDM1 rescue. Double knockdown experiments were also conducted in dTHP1 cells. The method of this experiment was referred to the previous research and 10 nM of each siRNA were transfected simultaneously (28).