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IPGTT, intraperitoneal insulin release test (IRT), and insulin tolerance test (ITT)
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WT and Kcnh6 KO mice and control and Kcnh6 βKO mice were assigned to weight- and sex-matched groups and received BBR (560 mg/kg body weight)11 or vehicle by gavage 1 hr before the intravenous injection of glucose to reach the maximum blood concentration of BBR. In some experiments, tolbutamide (40 mg/kg body weight) or vehicle were administered by gavage before the intravenous injection of glucose. The IPGTT, IRT, and ITT were performed as described previously8.
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