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Acquisition of Leftward Flow in Xenopus laevis   

Edited by
Ru Zhang
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Abstract

In Xenopus, the left-right axis is established following an extracellular vectorial leftward flow driven by monocilia at the gastrocoel roof plate (GRP) during late gastrulation / neurulation (Schweickert et al., 2007). As the GRP lies inside the developing archenteron, imaging of flow is challenging. Here we present the detailed procedure to visualize leftward flow in Xenopus laevis embryos.

Keywords: Cilia, Left-right asymmetry, Imaging, Leftward flow, Gastrocoel roof plate

Copyright: © 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Thumberger, T. and Blum, M. (2013). Acquisition of Leftward Flow in Xenopus laevis. Bio-protocol 3(23): e996. DOI: 10.21769/BioProtoc.996.
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