Acquisition of Leftward Flow in Xenopus laevis   

Edited by
Ru Zhang
Download PDF How to cite Favorites Q&A Share your feedback Cited by

In this protocol

Original research article

A brief version of this protocol appeared in:
Current Biology
Jan 2007


In Xenopus, the left-right axis is established following an extracellular vectorial leftward flow driven by monocilia at the gastrocoel roof plate (GRP) during late gastrulation / neurulation (Schweickert et al., 2007). As the GRP lies inside the developing archenteron, imaging of flow is challenging. Here we present the detailed procedure to visualize leftward flow in Xenopus laevis embryos.

Keywords: Cilia, Left-right asymmetry, Imaging, Leftward flow, Gastrocoel roof plate

Copyright: © 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Thumberger, T. and Blum, M. (2013). Acquisition of Leftward Flow in Xenopus laevis. Bio-protocol 3(23): e996. DOI: 10.21769/BioProtoc.996.

Please login to post your questions/comments. Your questions will be directed to the authors of the protocol. The authors will be requested to answer your questions at their earliest convenience. Once your questions are answered, you will be informed using the email address that you register with bio-protocol.
You are highly recommended to post your data including images for the troubleshooting.

You are highly recommended to post your data (images or even videos) for the troubleshooting. For uploading videos, you may need a Google account because Bio-protocol uses YouTube to host videos.