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Implantation of Dkk-1-soaked Beads into the Neural Tube of Chicken Embryos   

Edited by
Xuecai Ge
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Abstract

Chick embryos are known to be a powerful system to test gene function due to the “in vivo” accessibility, short time for results retrieval and the possibility to perform a large number of experiments. Synthetic micro-beads soaked in morphogenetic signals or receptor inhibitors can be implanted in selective embryo regions at precise developmental stages activating or blocking different signaling pathways. Here, we describe the manipulation of Wnt signaling pathway using Dkk-1-soaked micro-beads, implanted in ovo in the anterior part of the developing neural tube of chicken embryos; specifically at the prospective zona limitans intrathalamica at stage HH10.

Keywords: Experimental embryology, Neutral tube, Wnt signaling regulation, Microbeads implant, Ni vivo experimentos

Copyright: © 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite:  Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
  1. Martinez-Ferre, A., Navarro-Garberí, M., Bueno, C. and Martinez, S. (2013). Implantation of Dkk-1-soaked Beads into the Neural Tube of Chicken Embryos. Bio-protocol 3(21): e963. DOI: 10.21769/BioProtoc.963.
  2. Martinez-Ferre, A., Navarro-Garberi, M., Bueno, C. and Martinez, S. (2013). Wnt signal specifies the intrathalamic limit and its organizer properties by regulating Shh induction in the alar plate. J Neurosci 33(9): 3967-3980.    
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