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Measuring Oxygen Consumption Rate in Caenorhabditis elegans   

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Original research article

A brief version of this protocol appeared in:
Nature
May 2015

Abstract

The rate of oxygen consumption is a vital marker indicating cellular function during lifetime under normal or metabolically challenged conditions. It is used broadly to study mitochondrial function (Artal-Sanz and Tavernarakis, 2009; Palikaras et al., 2015; Ryu et al., 2016) or investigate factors mediating the switch from oxidative phosphorylation to aerobic glycolysis (Chen et al., 2015; Vander Heiden et al., 2009). In this protocol, we describe a method for the determination of oxygen consumption rates in the nematode Caenorhabditis elegans.

Keywords: Ageing, Caenorhabditis elegans, Metabolism, Mitochondria, Oxygen sensor, ROS

Background

Recent evidence underlines mitochondrial function as a potential contributor in the maintenance of organismal homeostasis and viability (Vafai and Mootha, 2012). Cellular oxygen consumption is highly recognized as a fundamental indicator of mitochondrial function, reflecting reactive oxygen species (ROS) production and metabolic activity of the cell. Therefore, several methods have been developed to measure oxygen consumption rates in cells or entire organisms (Dranka et al., 2011; Li and Graham, 2012; Luz et al., 2015; Perry et al., 2013). These approaches provided insight into the pivotal roles of mitochondria in disease progression and pathogenesis (Scheibye-Knudsen et al., 2015). In this protocol, we describe a method for the determination of oxygen consumption rates in the nematode C. elegans by using a Clark-type polarographic oxygen sensor electrode (Hansatech, King’s Lynn, England).

Copyright: © 2016 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Palikaras, K. and Tavernarakis, N. (2016). Measuring Oxygen Consumption Rate in Caenorhabditis elegans. Bio-protocol 6(23): e2049. DOI: 10.21769/BioProtoc.2049.
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