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Fat Turnover Assay in Drosophila   

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Original research article

A brief version of this protocol appeared in:
Cell Metabolism
Jan 2016

Abstract

Like all animals, Drosophila shows robust fat (triglyceride) turnover, i.e., they synthesize, store and utilize triglyceride for their daily metabolic needs. The protocol describes a simple assay to measure this turnover of triglycerides in Drosophila.

Background

Almost all animals store energy reserves in the form of glycogen and triglycerides. Many physiological, pathological and environmental conditions cause changes in the total level of these energy reserves, especially triglycerides. However, it’s not always clear whether the resulting changes in triglycerides are due to reduced breakdown, increased synthesis or vice versa. With this protocol, it is possible to determine both the rate of synthesis and degradation of the newly synthesized triglycerides in flies.

Copyright: © 2016 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Katewa, S. D. (2016). Fat Turnover Assay in Drosophila. Bio-protocol 6(21): e1996. DOI: 10.21769/BioProtoc.1996.
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