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[Bio101] Transfection of S2 Cell with DNA Using CellFectin Reagent

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Abstract

This method provides a step-by-step guide to transfecting Drosophila S2 cells with the pRmHA-3 (or similar) vector with insert of choice (in this case SDF-1β-FLAG) and generating a stable cell line. This cell line is then capable of producing the protein of interest under inducible conditions by addition of copper sulfate, which can then be purified and used as desired. This protocol provides an example to finding out when your peak protein production occurs and a method for determining optimal selection conditions.

How to cite: Cronshaw, D. G. (2012). Transfection of S2 Cell with DNA Using CellFectin Reagent. Bio-protocol Bio101: e190. DOI: 10.21769/BioProtoc.190.
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