Root-knot Nematode Penetration and Sclareol Nematicidal Activity Assays   

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Original research article

A brief version of this protocol appeared in:
Molecular Plant Microbe Interactions
Apr 2015


Plant parasitic nematodes parasitize roots and/or stems of various plants thereby inhibiting absorption of nutrients and moisture. In particular, root-knot nematodes (RKN) are a group of the most devastating pests. Various techniques, such as soil sterilization, cultivation of resistant crops, and chemical application, have been developed to control damage caused by RKN. Among these techniques, diminish by chemicals that induce or activate host defense to RKN is an attractive method because of its potential to reduce the environmental burden caused by crop protection. Sclareol, a diterpene, was identified as a chemical that induces resistance to RKN (Fujimoto et al., 2015). Here we provide a protocol for assessing the impact of sclareol on the penetration of RKNs into tomato and Arabidopsis roots and the direct nematicidal impact of the chemical on nematodes. This protocol can be used for other nematode resistance-inducing chemicals.

Keywords: Root-knot nematode, Nematicidal activity, Nematode penetration, Stain method

Copyright: © 2016 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Fujimoto, T., Mizukubo, T., Abe, H. and Seo, S. (2016). Root-knot Nematode Penetration and Sclareol Nematicidal Activity Assays. Bio-protocol 6(12): e1848. DOI: 10.21769/BioProtoc.1848.

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