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Computational Identification of MicroRNA-targeted Nucleotide-binding Site-leucine-rich Repeat Genes in Plants   

Edited by
Tie Liu
Reviewed by
Anonymous reviewer
Zhu-Qing ShaoZhu-Qing Shao*Yan-Mei ZhangYan-Mei Zhang*Bin WangBin Wang Jian-Qun ChenJian-Qun Chen  (*contributed equally to this work)
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Original research article

A brief version of this protocol appeared in:
Plant Physiology
Sep 2014

Abstract

Plant genomes harbor dozens to hundreds of nucleotide-binding site-leucine-rich repeat (NBS-LRR, NBS for short) type disease resistance genes (Shao et al., 2014; Zhang et al., 2015). Proper regulation of these genes is important for normal growth of plants by reducing unnecessary fitness costs in the absence of pathogen infection. Recent studies have revealed that microRNAs are involved in regulation of NBS genes in plants (Zhai et al., 2011; Shivaprasad et al., 2012). This protocol describes computational methods for the genome-wide identification of plant NBS genes potentially regulated by microRNAs.

Copyright: © 2015 The Authors; exclusive licensee Bio-protocol LLC.
How to cite:  Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
  1. Shao, Z., Zhang, Y., Wang, B. and Chen, J. (2015). Computational Identification of MicroRNA-targeted Nucleotide-binding Site-leucine-rich Repeat Genes in Plants. Bio-protocol 5(21): e1637. DOI: 10.21769/BioProtoc.1637.
  2. Shao, Z. Q., Zhang, Y. M., Hang, Y. Y., Xue, J. Y., Zhou, G. C., Wu, P., Wu, X. Y., Wu, X. Z., Wang, Q., Wang, B. and Chen, J. Q. (2014). Long-term evolution of nucleotide-binding site-leucine-rich repeat genes: understanding gained from and beyond the legume family. Plant Physiol 166(1): 217-234.
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