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Infection Assay of Cyst Nematodes on Arabidopsis Roots   

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Original research article

A brief version of this protocol appeared in:
Molecular Plant Microbe Interactions
Sep 2014

Abstract

Plant parasitic nematodes are devastating pests on many crops. Juveniles (J2) of cyst nematodes invade the roots to induce a syncytium. This feeding site is their only source of nutrients. Male nematodes leave the roots after the fourth molt to mate with females. The females stay attached to their syncytia throughout their life and produce hundreds of eggs, which are contained in their bodies. When the females die their bodies form the cysts, which protect the eggs. Cysts can survive for many years in the soil until favorable conditions induce hatching of the juveniles.
The beet cyst nematode Heterodera schachtii is a pathogen of sugar beet (Beta vulgaris) but can also complete its life cycle on Arabidopsis roots growing on agar plates under sterile conditions. We present here protocols for a stock culture of H. schachtii and an infection assay on agar plates.

Keywords: Nematode infection assay, Cyst nematode, Heterodera schachtii, Arabidopsis

Copyright: © 2015 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Bohlmann, H. and Wieczorek, K. (2015). Infection Assay of Cyst Nematodes on Arabidopsis Roots. Bio-protocol 5(18): e1596. DOI: 10.21769/BioProtoc.1596.
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