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Protein Extraction from Drosophila Embryos and Ovaries   

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Original research article

A brief version of this protocol appeared in:
eLIFE
Apr 2014

Abstract

Here we provide the description of protocols to efficiently obtain protein extracts from embryos and ovaries of Drosophila melanogaster. These protocols are routinely applied in our laboratory and are based on two techniques: either embryos or ovaries are homogenized using a pestle and then the soluble proteins separated by centrifugation, or embryos are individually lysed by needle manipulation. The latter technique allows the use of small embryo numbers and the selection of specific developmental stages (Guilgur et al., 2014).

Keywords: Drosophila, Protein extracts, Embryos, Ovaires

Copyright Prudêncio and Guilgur. This article is distributed under the terms of the Creative Commons Attribution License (CC BY 4.0).
How to cite:  Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
  1. Prudêncio, P. and Guilgur, L. G. (2015). Protein Extraction from Drosophila Embryos and Ovaries. Bio-protocol 5(9): e1459. DOI: 10.21769/BioProtoc.1459.
  2. Guilgur, L. G., Prudencio, P., Sobral, D., Liszekova, D., Rosa, A. and Martinho, R. G. (2014). Re-quirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development. Elife 3: e02181.
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