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    Protocols in Current Issue
    Lipidomic Analysis of Caenorhabditis elegans Embryos
    Authors:  Hung-Chi Yang, Cheng-Yu Hung, Yi-Yun Pan, Szecheng J Lo and Daniel Tsun-Yee Chiu, date: 09/20/2017, view: 31, Q&A: 0
    Metabolomic is an emerging field of system biology. Lipidomic, a branch of metabolomic, aims to characterize lipophilic metabolites in biological systems. Caenorhabditis elegans (C. elegans) is a genetically tractable and versatile ...
    Lipidomic Analysis of Caenorhabditis elegans Embryos
    Authors:  Hung-Chi Yang, Cheng-Yu Hung, Yi-Yun Pan, Szecheng J Lo and Daniel Tsun-Yee Chiu, date: 09/20/2017, view: 31, Q&A: 0
    [Abstract] Metabolomic is an emerging field of system biology. Lipidomic, a branch of metabolomic, aims to characterize lipophilic metabolites in biological systems. Caenorhabditis elegans (C. elegans) is a genetically tractable and versatile animal model for novel discovery of lipid metabolism. In addition, C. elegans embryo is ...
    Superoxide Dismutase (SOD) and Catalase (CAT) Activity Assay Protocols for Caenorhabditis elegans
    Authors:  Jing Zhang, Rui Chen, Zhenyang Yu and Lili Xue, date: 08/20/2017, view: 388, Q&A: 0
    [Abstract] Assays for superoxide dismutase (SOD) and catalase (CAT) activities are widely employed to indicate antioxidant responses underlying the toxic effects of test chemicals. Yet, earlier studies mainly described the procedures as performed according to manufacturer’s instructions without modifications that are specific to any organisms. The present ...
    Primary Culture System for Germ Cells from Caenorhabditis elegans Tumorous Germline Mutants
    Authors:  Alexandra S. Vagasi, Mohammad M. Rahman, Snehal N. Chaudhari and Edward T. Kipreos, date: 08/05/2017, view: 382, Q&A: 0
    [Abstract] The Caenorhabditis elegans germ line is an important model system for the study of germ stem cells. Wild-type C. elegans germ cells are syncytial and therefore cannot be isolated in in vitro cultures. In contrast, the germ cells from tumorous mutants can be fully cellularized and isolated intact from the mutant animals. ...
    Separation and Purification of Glycosaminoglycans (GAGs) from Caenorhabditis elegans
    Authors:  Tabea Dierker and Lena Kjellén, date: 08/05/2017, view: 407, Q&A: 0
    [Abstract] The nematode Caenorhabditis elegans is a popular model organism for studies of developmental biology, neurology, ageing and other fields of basic research. Because many developmental processes are regulated by glycosaminoglyans (GAGs) on cell surfaces and in the extracellular matrix, methods to isolate and analyze C. elegans GAGs ...
    Aldicarb-induced Paralysis Assay to Determine Defects in Synaptic Transmission in Caenorhabditis elegans
    Authors:  Kelly H. Oh and Hongkyun Kim, date: 07/20/2017, view: 422, Q&A: 0
    [Abstract] Aldicarb treatment causes an accumulation of acetylcholine in the synaptic cleft of the neuromuscular junction, resulting in sustained muscle activation and eventually paralysis. Aldicarb-induced paralysis assay is an easy and fast method to determine whether synaptic transmission of a C. elegans mutant of interest is altered. This assay ...
    Oxidative Stress Assays (arsenite and tBHP) in Caenorhabditis elegans
    Authors:  Collin Yvès Ewald, John M. Hourihan and T. Keith Blackwell, date: 07/05/2017, view: 750, Q&A: 0
    [Abstract] Cells and organisms face constant exposure to reactive oxygen species (ROS), either from the environment or as a by-product from internal metabolic processes. To prevent cellular damage from ROS, cells have evolved detoxification mechanisms. The activation of these detoxification mechanisms and their downstream responses represent an overlapping ...
    Culturing Bacteria from Caenorhabditis elegans Gut to Assess Colonization Proficiency
    [Abstract] Determining an accurate count of intestinal bacteria from Caenorhabditis elegans is one critical way to assess colonization proficiency by a given bacteria. This can be accomplished by culturing appropriate dilutions of worm gut bacteria on selective or differential agarized media. Because of the high concentration of bacteria in gut ...
    Single-molecule RNA Fluorescence in situ Hybridization (smFISH) in Caenorhabditis elegans
    [Abstract] Single-molecule RNA fluorescence in situ hybridization (smFISH) is a technique to visualize individual RNA molecules using multiple fluorescently-labeled oligonucleotide probes specific to the target RNA (Raj et al., 2008; Lee et al., 2016a). We adapted this technique to visualize RNAs in the C. elegans whole ...
    Heavy Metal Stress Assay of Caenorhabditis elegans
    Authors:  Strahil Iv. Pastuhov, Tatsuhiro Shimizu and Naoki Hisamoto, date: 06/05/2017, view: 865, Q&A: 0
    [Abstract] Organisms have developed many protective systems to reduce the toxicity from heavy metals. The nematode Caenorhabditis elegans has been widely used to determine the protective mechanisms against heavy metals. Responses against heavy metals can be monitored by expression of reporter genes, while sensitivity can be determined by quantifying ...
    Imaging the Pharynx to Measure the Uptake of Doxorubicin in Caenorhabditis elegans
    [Abstract] Caenorhabditis elegans offers an array of advantages to investigate the roles of uptake transporters. Herein, an epifluorescent microscopy approach was developed to monitor the uptake of the autofluorescent anticancer drug, doxorubicin, into the pharynx of C. elegans by organic cation transporters.